|
| Status |
Public on Jan 24, 2024 |
| Title |
AKB6, MRTX1133, ROI 007 |
| Sample type |
SRA |
| |
|
| Source name |
AKB6
|
| Organism |
Mus musculus |
| Characteristics |
cell line: AKB6
genotype: KRAS G12D
treatment: MRTX1133
|
| Treatment protocol |
Mice were treated with MRTX1133 once per day for three weeks. The drug was administed intraperitoneally . MRTX1133 was dissolved in 50 mM citrate buffer and 10% Captisol. The control mice received the vehicle (50 mM citrate buffer and 10% Captisol) treatement, which was adminsitered intraperitoneally.
|
| Growth protocol |
AKB6 cell line cultured in RPMI media supplemented with 10% FBS and were maintained in 37°C with 5% CO2. The xenografts were developed in C57BL/6 strain by subcutaneously injecting 500,000 cells/mouse.The mice were always maintained in Doxycycline water to induce the oncogenic KRAS
|
| Extracted molecule |
other |
| Extraction protocol |
RNAs with barcode are prepared using GeoMx DSP instrument, nanoString, Inc.
|
| |
|
| Library strategy |
OTHER |
| Library source |
other |
| Library selection |
other |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
g2_ROI_007
|
| Data processing |
GeoMx_NGS_Pipeline_2.3.3.10
Assembly: Mm_R_NGS_WTA_v1.0.pkc
Supplementary files format and content: DCC files: count data for each barcode.
Supplementary files format and content: Sample_Annotation.xlsx
|
| |
|
| Submission date |
Dec 06, 2023 |
| Last update date |
Jan 24, 2024 |
| Contact name |
Jianxin Wang |
| Organization name |
Roswell Park Comprehensive Cancer Center
|
| Department |
Molecular and Cellular Biology
|
| Street address |
665 Elm Street
|
| City |
Buffalo |
| State/province |
NY |
| ZIP/Postal code |
14203 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (2) |
| GSE249538 |
Extracellular niche and tumor microenvironment enhance the impact of KRAS inhibitors in pancreatic cancer models [DSP] |
| GSE249541 |
Extracellular niche and tumor microenvironment enhance the impact of KRAS inhibitors in pancreatic cancer models. |
|
| Relations |
| BioSample |
SAMN38699612 |
| SRA |
SRX22806498 |
