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Sample GSM7949463 Query DataSets for GSM7949463
Status Public on Jan 24, 2024
Title AKB6, MRTX1133, ROI 006
Sample type SRA
 
Source name AKB6
Organism Mus musculus
Characteristics cell line: AKB6
genotype: KRAS G12D
treatment: MRTX1133
Treatment protocol Mice were treated with MRTX1133 once per day for three weeks. The drug was administed intraperitoneally . MRTX1133 was dissolved in 50 mM citrate buffer and 10% Captisol. The control mice received the vehicle (50 mM citrate buffer and 10% Captisol) treatement, which was adminsitered intraperitoneally.
Growth protocol AKB6 cell line cultured in RPMI media supplemented with 10% FBS and were maintained in 37°C with 5% CO2. The xenografts were developed in C57BL/6 strain by subcutaneously injecting 500,000 cells/mouse.The mice were always maintained in Doxycycline water to induce the oncogenic KRAS
Extracted molecule other
Extraction protocol RNAs with barcode are prepared using GeoMx DSP instrument, nanoString, Inc.
 
Library strategy OTHER
Library source other
Library selection other
Instrument model Illumina NovaSeq 6000
 
Description g2_ROI_006
Data processing GeoMx_NGS_Pipeline_2.3.3.10
Assembly: Mm_R_NGS_WTA_v1.0.pkc
Supplementary files format and content: DCC files: count data for each barcode.
Supplementary files format and content: Sample_Annotation.xlsx
 
Submission date Dec 06, 2023
Last update date Jan 24, 2024
Contact name Erik Knudsen
E-mail(s) Erik.Knudsen@RoswellPark.org
Organization name Roswell Park Comprehensive Cancer Center
Department Molecular and Cellular Biology
Street address 665 Elm Street
City Buffalo
State/province NY
ZIP/Postal code 14203
Country USA
 
Platform ID GPL24247
Series (2)
GSE249538 Extracellular niche and tumor microenvironment enhance the impact of KRAS inhibitors in pancreatic cancer models [DSP]
GSE249541 Extracellular niche and tumor microenvironment enhance the impact of KRAS inhibitors in pancreatic cancer models.
Relations
BioSample SAMN38699613
SRA SRX22806497

Supplementary file Size Download File type/resource
GSM7949463_DSP-1001660011347-H-A10.dcc.gz 67.2 Kb (ftp)(http) DCC
SRA Run SelectorHelp
Raw data are available in SRA

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