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Sample GSM7947045 Query DataSets for GSM7947045
Status Public on Dec 06, 2023
Title ductal_KPTS_cultured_RNAseq_3
Sample type SRA
Source name exocrine pancreas
Organism Homo sapiens
Characteristics tissue: exocrine pancreas
cell type: ductal
genotype: KPTS
condition: monolayer culture
Treatment protocol "K" genotype cells have enforced expression of oncogenic KRAS-G12V and "KPTS" genotype cells have KRAS-G12V and CRISPR/Cas9 knockout of P16, P53, and SMAD4 .
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Zymo Direct-zol RNA Miniprep Kit as per manufacturer instructions.
RNAseq libraries prepared using Illumina Stranded mRNA Prep Ligation kit as per manufacturer instructions.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 3000
Data processing Alignment was performed using TopHat 2.1.1 and gene expression was quantified using HTSeq 0.11.1.
Assembly: hg38
Supplementary files format and content: read_table.txt.gz is tab delimited table of read counts per gene for all samples.
Supplementary files format and content: TPM_table.txt.gz is tab delimited table of transcripts per million per gene for all samples
Submission date Dec 05, 2023
Last update date Dec 06, 2023
Contact name Pei Wang
Organization name University of Texas Health San Antonio
Department Cell Systems & Anatomy
Street address 7703 Floyd Curl Dr
City San Antonio
State/province TX
ZIP/Postal code 78229
Country USA
Platform ID GPL21290
Series (2)
GSE222990 RNA sequencing of human pancreatic acinar and ductal cells
GSE222991 Functional Genomics of human pancreatic acinar and ductal cells
BioSample SAMN38685814
SRA SRX22789556

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA

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