|Public on Dec 06, 2023
|tissue: exocrine pancreas
cell type: ductal
condition: monolayer culture
|"K" genotype cells have enforced expression of oncogenic KRAS-G12V and "KPTS" genotype cells have KRAS-G12V and CRISPR/Cas9 knockout of P16, P53, and SMAD4 .
|Total RNA was extracted using Zymo Direct-zol RNA Miniprep Kit as per manufacturer instructions.
RNAseq libraries prepared using Illumina Stranded mRNA Prep Ligation kit as per manufacturer instructions.
|Illumina HiSeq 3000
|Alignment was performed using TopHat 2.1.1 and gene expression was quantified using HTSeq 0.11.1.
Supplementary files format and content: read_table.txt.gz is tab delimited table of read counts per gene for all samples.
Supplementary files format and content: TPM_table.txt.gz is tab delimited table of transcripts per million per gene for all samples
|Dec 05, 2023
|Last update date
|Dec 06, 2023
|University of Texas Health San Antonio
|Cell Systems & Anatomy
|7703 Floyd Curl Dr
|RNA sequencing of human pancreatic acinar and ductal cells
|Functional Genomics of human pancreatic acinar and ductal cells