GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Sample GSM7927228 Query DataSets for GSM7927228
Status Public on Mar 13, 2024
Title Pla2g3-/-, antigen challenged, bronchial epithelial cells
Sample type RNA
Source name Pla2g3-/-, antigen challenged, bronchial epithelial cells
Organism Mus musculus
Characteristics tissue: bronchial epithelial cells
genotype: Pla2g3-/-
treatment: antigen challenged
Treatment protocol 3 h after antigen challenge, lungs were harvested, and bronchial epithelial cells were isolated from lung tissues (reference to Kuroishi S et al. Respirology 14, 828-837, 2009) for microarray analysis.
Growth protocol Mice were sensitized with intraperitoneal injections of OVA (Grade V, Merk-Sigma-Aldrich) (10 µg) as a antigen plus 1.3 mg of aluminum hydroxide (Alu-Gel-S suspension, SERVA) (alum adjuvant) in 0.2 ml of saline on days 0 and 14. On days 26, 27, and 28, the mice were challenged with 1% OVA aeroallergen for 30 min per day.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from cells and purified, and the quality of RNA was assessed with a 2100 Bioanalyzer (Agilent Technologies).
Label Cy3
Label protocol 200 ng of total RNA was amplified and labeled using the Low Input Quick Amp Labeling Kit (Agilent Technologies) according to the manufacturer's protocol.
Hybridization protocol 1.65 µg of Cy3-labeled cRNA was fragmented using the Agilent Gene Expression Hybridization Kit and hybridized with a Whole Mouse Genome Microarray (4x44K, Agilent Technologies). Hybridizations were performed for 17 hours at 65ºC at 10 rpm in a rotating hybridization oven.
Scan protocol The slides were scanned immediately after washing on SureScan Microarray Scanner (Agilent Technologies) using one color scan setting for 4x44k array slides (Scan resolution 5 um, Dye channel is set to Green and Green PMT is set to 100%).
Data processing Data was obtained using the Agilent Feature Extraction software (v11.5.1.1) with set to default for all parameters.
Submission date Dec 01, 2023
Last update date Mar 13, 2024
Contact name Yoshitaka Taketomi
Organization name The University of Tokyo
Department CDBIM, Graduate School of Medicine,
Lab Laboratory of Microenvironmental Metabolic Health Science
Street address 7-3-1 Hongo, Bunkyo-ku,
City Tokyo
ZIP/Postal code 113-8655
Country Japan
Platform ID GPL10333
Series (1)
GSE249135 Effect of group III phospholipase A2 deletion on gene expression in bronchial epithelial cells in an antigen-induced asthma model.

Data table header descriptions
VALUE The microarray data were analyzed using GeneSpring GX software (Agilent Technologies). Normalized signal intensity (Shift to 75 percentile).

Data table
1 8.103029078
2 0.001714586
3 0.001720266
4 0.001725172
5 0.001729427
6 0.001732892
7 0.001735758
8 0.001737901
9 0.001739493
10 0.001740548
11 0.001741041
12 0.100367228
13 0.003509908
14 5.96088725
15 0.029068201
16 3.035813354
17 28.40970113
18 0.001731489
19 0.011730935
20 84.29932042

Total number of rows: 44397

Table truncated, full table size 764 Kbytes.

Supplementary file Size Download File type/resource
GSM7927228_SG12334215_252665518475_S001_GE1_1105_Oct12_1_4.txt.gz 8.9 Mb (ftp)(http) TXT

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap