|
| Status |
Public on Mar 01, 2024 |
| Title |
MM1144, control, H3K27ac ChIP, biorep2 |
| Sample type |
SRA |
| |
|
| Source name |
Haematopoietic and Lymphoid
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Haematopoietic and Lymphoid
cell line: MM.1-144
cell type: Plasma Cell Myeloma
genotype: WT
Sex: Female
treatment: control
chip antibody: H3K27ac antibody (07-360; Merck)
|
| Treatment protocol |
Cells were transduced with TLCV2-containing virus and selected with 0.5 µg/ml puromycin for 72H. Transduced cells were subjected to 1 µg/ml Doxycycline to induce Cas9 expression before GFP sorting.
|
| Growth protocol |
Cells were cultured in RPMI 1640 media with 2.05 mM L-Glutamine (SH30027.01; Hyclone).
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cells were fixed in 1% formaldehyde for 12 minutes, swelled in 0.5% SDS buffer then lysed in 0.5% SDS + 5% Triton buffer (2:1). Genomic DNA was sheared to 100-500bp by sonication.
ChIP libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB).
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Reads aligned to hg38 using bwa mem and filtered for duplicates, poor quality and blacklisted regions
Coverage across 20 bp windows quantified by BAMscale
Peaks called by MACS2
Assembly: hg38
Supplementary files format and content: bigWig, fragment coverage across 20 bp windows
Supplementary files format and content: narrowPeak, MACS2 peak calls
|
| |
|
| Submission date |
Dec 01, 2023 |
| Last update date |
Mar 01, 2024 |
| Contact name |
Yinghui Li |
| E-mail(s) |
liyh@ntu.edu.sg
|
| Organization name |
Nanyang Technological University
|
| Department |
School of Biological Sciences
|
| Lab |
05n-21
|
| Street address |
60 Nanyang Drive
|
| City |
Singapore |
| State/province |
Singapore |
| ZIP/Postal code |
637551 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL24676 |
| Series (2) |
| GSE230466 |
Aberrant non-canonical NF-κB signalling reprograms the epigenome landscape to drive oncogenic transcriptomes in multiple myeloma [ChIP-seq] |
| GSE230526 |
Aberrant non-canonical NF-κB signalling reprograms the epigenome landscape to drive oncogenic transcriptomes in multiple myeloma |
|
| Relations |
| BioSample |
SAMN38562470 |
| SRA |
SRX22705700 |
