|
| Status |
Public on Jun 01, 2024 |
| Title |
cko4 |
| Sample type |
SRA |
| |
|
| Source name |
Bone marrow
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: Bone marrow
cell type: HSCs
genotype: Nynrin CKO
age: 8 weeks old
|
| Treatment protocol |
Nynrinfl/flTie2-Cre and Nynrinfl/fl mice
|
| Growth protocol |
HSCs isolated from BM of mice
|
| Extracted molecule |
total RNA |
| Extraction protocol |
About 3000 HSCs of BMs from Nynrinfl/flTie2-Cre or Nynrinfl/fl mice were sorted by FACS,and extraction and Purification of total RNA using Trizol. 5 ng cDNA was used for the construction of sequencing libraries.
The construct of the whole cDNA library for low number HSCs from from Nynrin cko mice was performed as previously described (Chen et al., 2017, PMID: 28207000).
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
CKO4
|
| Data processing |
Nynrinfl/flTie2-Cre vs Nynrinfl/fl mice
FPKM of differential genes
Assembly: mm10
Supplementary files format and content: Raw_counts_expression.txt
|
| |
|
| Submission date |
Nov 26, 2023 |
| Last update date |
Jun 01, 2024 |
| Contact name |
CHENGFANG ZHOU |
| E-mail(s) |
chengfangzhou47@gmail.com
|
| Phone |
(86)13368348471
|
| Organization name |
Chongqing Medical University
|
| Department |
Institute of Life Sciences
|
| Street address |
No. 1, Medical College Road
|
| City |
Chongqing |
| ZIP/Postal code |
400032 |
| Country |
China |
| |
|
| Platform ID |
GPL24247 |
| Series (2) |
| GSE248637 |
Nynrin preserves hematopoietic stem cell function by inhibiting the mitochondrial permeability transition pore opening (RNA-Seq for Nynrin cko HSC) |
| GSE248639 |
Nynrin preserves hematopoietic stem cell function by inhibiting the mitochondrial permeability transition pore opening. |
|
| Relations |
| BioSample |
SAMN38440423 |
| SRA |
SRX22643584 |
