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Sample GSM7910319 Query DataSets for GSM7910319
Status Public on Jan 15, 2024
Title IgD- Ighg2A10, WT_CSP9, Transcriptome
Sample type SRA
 
Source name IgD- Ighg2A10, WT_CSP9
Organism Mus musculus
Characteristics cell type: Mouse B cells
Extracted molecule total RNA
Extraction protocol Mouse splenocytes were incubated with CITE-seq antibodies and flow cytometry antibodies, washed, and resuspend in sorting buffer (PBS+2% FBS) for sorting IgD- Ighg2A10 B cells. The sorted cells were pooled into 4 samples for library construction according to protocols by 10X Genomics
Library was performed according to 10X Genomic's instructions
scRNA-seq, CITE-seq, VDJ-seq
 
Library strategy OTHER
Library source transcriptomic
Library selection other
Instrument model Illumina NovaSeq 6000
 
Description Sample3_WT_CSP9_Transcriptome
Data processing Data processing was performed by CellRanger V6.0 under default settings
Assembly: mm10
Supplementary files format and content: Sample1_MD4_CSP9_Processed.tar.gz
Supplementary files format and content: Sample2_MD4_CSP27_Processed.tar.gz
Supplementary files format and content: Sample3_WT_CSP9_Processed.tar.gz
Supplementary files format and content: Sample4_WT_CSP27_Processed.tar.gz
 
Submission date Nov 20, 2023
Last update date Jan 15, 2024
Contact name Xin Gao
E-mail(s) xin.gao@anu.edu.au
Phone 0450031169
Organization name The Australian National University
Department Immunology
Street address 131 Garran Rd
City Canberra
State/province ACT
ZIP/Postal code 2601
Country Australia
 
Platform ID GPL24247
Series (2)
GSE248255 Lack of affinity signature for germinal center cells that have initiated plasma cell differentiation (Figure4)
GSE248377 Lack of affinity signature for germinal center cells that have initiated plasma cell differentiation
Relations
BioSample SAMN38334411
SRA SRX22585251

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA

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