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Status |
Public on Jan 15, 2024 |
Title |
IgD- Ighg2A10, MD4_CSP9, VDJ |
Sample type |
SRA |
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Source name |
IgD- Ighg2A10, MD4_CSP9
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Organism |
Mus musculus |
Characteristics |
cell type: Mouse B cells
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Extracted molecule |
total RNA |
Extraction protocol |
Mouse splenocytes were incubated with CITE-seq antibodies and flow cytometry antibodies, washed, and resuspend in sorting buffer (PBS+2% FBS) for sorting IgD- Ighg2A10 B cells. The sorted cells were pooled into 4 samples for library construction according to protocols by 10X Genomics Library was performed according to 10X Genomic's instructions scRNA-seq, CITE-seq, VDJ-seq
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Library strategy |
OTHER |
Library source |
transcriptomic |
Library selection |
other |
Instrument model |
Illumina NovaSeq 6000 |
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Description |
Sample1_MD4_CSP9_VDJ
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Data processing |
Data processing was performed by CellRanger V6.0 under default settings Assembly: mm10 Supplementary files format and content: Sample1_MD4_CSP9_Processed.tar.gz Supplementary files format and content: Sample2_MD4_CSP27_Processed.tar.gz Supplementary files format and content: Sample3_WT_CSP9_Processed.tar.gz Supplementary files format and content: Sample4_WT_CSP27_Processed.tar.gz
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Submission date |
Nov 20, 2023 |
Last update date |
Jan 15, 2024 |
Contact name |
Xin Gao |
E-mail(s) |
xin.gao@anu.edu.au
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Phone |
0450031169
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Organization name |
The Australian National University
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Department |
Immunology
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Street address |
131 Garran Rd
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City |
Canberra |
State/province |
ACT |
ZIP/Postal code |
2601 |
Country |
Australia |
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Platform ID |
GPL24247 |
Series (2) |
GSE248255 |
Lack of affinity signature for germinal center cells that have initiated plasma cell differentiation (Figure4) |
GSE248377 |
Lack of affinity signature for germinal center cells that have initiated plasma cell differentiation |
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Relations |
BioSample |
SAMN38334418 |
SRA |
SRX22585244 |
Supplementary data files not provided |
SRA Run Selector |
Raw data are available in SRA |
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