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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 01, 2024 |
| Title |
Lung, LUAD off DOX, time 2, pooled |
| Sample type |
SRA |
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| Source name |
Lung
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| Organism |
Mus musculus |
| Characteristics |
tissue: Lung
genotype: AT2-ERPMT
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| Treatment protocol |
vehicle or osimertinib
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| Growth protocol |
all cells were freshly isolated from lungs of normal or tumorigenic mice
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| Extracted molecule |
total RNA |
| Extraction protocol |
10X Chromium 5' single cell RNA v3 chemistry protocol
10X Genomics Chromium Next GEM Single Cell 3' Reagent Kits, v3.1 Dual Index Chemistry
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
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| Data processing |
Single cell RNA-seq reads were aligned, demultiplexed, and processed using CellRanger (v5.0.1). Single cells libraries were filtered for high quality cells based on cell size, mitochondrial content, doublet score, and library complexity.
Assembly: mm10, plus TdTom and Tg.EGFR transgenes
Supplementary files format and content: .h5ad: anndata object for single cell RNA-seq experiments. Contains normalize, log-transformed, and imputed counts, along with cell and gene level feature information.
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| Submission date |
Nov 19, 2023 |
| Last update date |
Jan 01, 2024 |
| Contact name |
Ashley M Laughney |
| E-mail(s) |
ashley.laughney@gmail.com
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| Organization name |
Weill Cornell Medicine
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| Department |
Physiology, Biophysics, and Systems Biology
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| Lab |
Laughney Lab
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| Street address |
413 East 69th Street
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| City |
NEW YORK |
| State/province |
New York |
| ZIP/Postal code |
10021 |
| Country |
USA |
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| Platform ID |
GPL24247 |
| Series (2) |
| GSE248202 |
Lineage-specific intolerance to oncogenic drivers restricts histological transformation [scRNA-Seq] |
| GSE248207 |
Lineage-specific intolerance to oncogenic drivers restricts histological transformation |
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| Relations |
| BioSample |
SAMN38320988 |
| SRA |
SRX22573329 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
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