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Sample GSM7906203 Query DataSets for GSM7906203
Status Public on Jan 24, 2024
Title control,Ngn2,AMG,CMO308,Day 20
Sample type SRA
 
Source name cel line
Organism Homo sapiens
Characteristics tissue: cel line
cell line: HMGU-1
cell type: pAstro
genotype: control
transcription factor: Ngn2
treatment: AMG
day: Day 20
barcode (10x): CMO308
Treatment protocol AMG was added to the medium, when needed
Growth protocol pAstros from different genotypes were transduced with the indicated retroviruses; 2 days post transduction, the medium was changed with differentiation medium.
Extracted molecule polyA RNA
Extraction protocol Cells were collected at 20 days post transduction and FACS sorted, each sample into a separate tube. Then, CMO solution was added to the sorted cells, according to manifacturer's protocol (10XGenomics).
Equal number of cells from each condition was added to have a correct cell concentration. Then, ~20000 cells were loaded per lane, and 2 lanes were loaded.
Libraries were prepared according to manifacturer's protocol (10XGenomics).
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description 10XGenomics
Data processing Raw data were processed using CellRanger 6.0, according to manifacturer's protocol (10XGenomics).
Assembly: hg28, ensembl98
Supplementary files format and content: tsv file format
 
Submission date Nov 17, 2023
Last update date Jan 24, 2024
Contact name Giacomo Masserdotti
E-mail(s) giacomo.masserdotti@helmholtz-munich.de
Organization name Helmholtz Zentrum
Department ISF
Street address Groshaderner Strasse 9
City Planegg-Martinstied
ZIP/Postal code 82152
Country Germany
 
Platform ID GPL24676
Series (2)
GSE248128 Unfolded protein Response is a major hurdle in direct neuronal reprogramming of human astrocytes [scRNA-seq day 20]
GSE248129 Unfolded protein Response is a major hurdle in direct neuronal reprogramming of human astrocytes

Supplementary data files not provided
Raw data are available in SRA

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