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Sample GSM7906183 Query DataSets for GSM7906183
Status Public on Jan 24, 2024
Title control,Ngn2,no treatment,CMO306,Day 5
Sample type SRA
Source name cel line
Organism Homo sapiens
Characteristics tissue: cel line
cell line: HMGU-1
cell type: pAstro
genotype: control
transcription factor: Ngn2
treatment: no treatment
day: Day 5
barcode (10x): CMO306
Treatment protocol AMG was added to the medium, when needed
Growth protocol pAstros from different genotypes were transduced with the indicated retroviruses; 48 hours later, the medium was changed with differentiation medium.
Extracted molecule polyA RNA
Extraction protocol Cells were collected and FACS sorted at 5 days post infection, each sample into a separate tube. Then, CMO solution was added to the cells, according to manifacturer's protocol (10XGenomics).
Equal number of cells from each condition was added to have a correct cell concentration. Then, ~20000 cells were loaded per lane, and 2 lanes were loaded.
Libraries were prepared according to manifacturer's protocol (10XGenomics).
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
Description 10XGenomics
Data processing Raw data were processed using CellRanger 6.0, according to manifacturer's protocol (10XGenomics).
Assembly: hg28, ensembl98
Supplementary files format and content: tsv file format
Submission date Nov 17, 2023
Last update date Jan 24, 2024
Contact name Giacomo Masserdotti
Organization name Helmholtz Zentrum
Department ISF
Street address Groshaderner Strasse 9
City Planegg-Martinstied
ZIP/Postal code 82152
Country Germany
Platform ID GPL24676
Series (2)
GSE248122 Unfolded protein Response is a major hurdle in direct neuronal reprogramming of human astrocytes [scRNA-seq 5 day]
GSE248129 Unfolded protein Response is a major hurdle in direct neuronal reprogramming of human astrocytes

Supplementary data files not provided
Raw data are available in SRA

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