|
Status |
Public on Mar 01, 2024 |
Title |
week 2 aristolochic acid_FB |
Sample type |
SRA |
|
|
Source name |
kidney
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 tissue: kidney cell type: Cd45+ FACS immune cells gender: male disease state: Chronic kidney disease/ transition of acute kidney injury-to-chronic kidney disease
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Cells were isolated, tagged with hashing antibodies, and combined (week 2 samples combined and week 6 samples combined separately) for multiplex sequencing, and sequenced per 10x genomics instruction.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic single cell |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
10X Genomics W2_1 was B0301, W2_2 was B0302, W2_3 was B0303
|
Data processing |
Libraries were multiplex sequenced and processed following 10X Genomics protocol for CellRanger. Assembly: mm10
|
|
|
Submission date |
Nov 01, 2023 |
Last update date |
Mar 01, 2024 |
Contact name |
Kyle Hartley Moore |
E-mail(s) |
kylemoore@uabmc.edu
|
Organization name |
University of Alabama at Birmingham
|
Department |
Division of Nephrology
|
Lab |
Anupam Agarwal
|
Street address |
703 19th St. S.
|
City |
Birmingham |
State/province |
Alabama |
ZIP/Postal code |
35294 |
Country |
USA |
|
|
Platform ID |
GPL24247 |
Series (1) |
GSE246803 |
Cognate antigen-independent differentiation of resident memory t cells in chronic kidney disease |
|
Relations |
BioSample |
SAMN38066139 |
SRA |
SRX22333080 |