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| Status |
Public on Jul 17, 2024 |
| Title |
WT biol rep 3 |
| Sample type |
SRA |
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| Source name |
primary cortical neurons
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| Organism |
Mus musculus |
| Characteristics |
cell type: primary cortical neurons
genotype: WT
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
Zymo Quick-RNA Minprep kits were used to isolate RNA
RNA libraries were prepared for sequencing using TruSeq stranded mRNA library prep kit
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 1000 |
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| Data processing |
Fastq files were aligned by Star
DESeq2 was used for differential gene expression analysis
Assembly: mm10
Supplementary files format and content: csv of DESeq output
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| Submission date |
Oct 31, 2023 |
| Last update date |
Jul 17, 2024 |
| Contact name |
Emily Feierman |
| E-mail(s) |
emily.feierman@gmail.com
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| Organization name |
University of Pennsylvania
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| Street address |
3400 Civic Center Blvd
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| City |
Philadelphia |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platform ID |
GPL32159 |
| Series (2) |
| GSE246686 |
RNA-seq analysis of WT and H2BE-KO primary cortical neurons |
| GSE246688 |
CUT&Tag, ATAC-seq, bulk RNA-seq and single-nucleus RNA-seq analysis of histone variant H2BE in cortical neurons |
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| Relations |
| BioSample |
SAMN38053548 |
| SRA |
SRX22323622 |
| Supplementary data files not provided |
SRA Run Selector |
| Raw data are available in SRA |
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