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Sample GSM7865084 Query DataSets for GSM7865084
Status Public on Jun 16, 2024
Title Healthy control,7w, biological replication 1
Sample type SRA
 
Source name Liver
Organism Mus musculus
Characteristics tissue: Liver
strain: C57BL/6J
age: 7 weeks
streptozotocin treatment: Untreated
diet: SCD
Sex: Male
genotype: WT
drug treatment: Untreated
Treatment protocol Vehicle (HyClone DPBS, Cytiva, Germany) or tirzepatide (109.5 nmol/kg, CPC, Hanzhou, China) dissolved in vehicle was administered via subcutaneous injection to ad libitum-fed STZ+HFD mice 1-2 hours prior to the onset of the dark cycle every 2 days for 10 to 11 weeks.
Growth protocol Mice were housed in climate-controlled, specific pathogen-free barrier facilities under a 12 h light-dark cycle and provided diet and water ad libitum. They were fed standard chow diet (SCD) (Teklad Global 18% Protein Rodent Diet 2018; Harlan Teklad, WI, USA), 18% fat or HFD (Research Diet (New Brunswick, NJ, USA) D12492, 60% fat) from 8 weeks of age.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using a RNeasy Mini kit (Qiagen, Hilden, Germany; 74104) in keeping with the manufacturer’s instructions.
The RNA sequencing library was prepared using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswitch, MA, USA; E7760).
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description rawcount_allsamples_mouse.txt
Batch1_Control07w_1
Data processing Raw RNA-sequencing data obtained from mouse were mapped to mouse genome build mm10 (GRCm38) and those from human mapped to human genome build 38 (GRCh38) using STAR (version 020201).
Count matrices were generated using RSEM (v1.3.1)
Assembly: mm10, human genome build 38 (GRCh38)
Supplementary files format and content: tab-delimited text files include raw expected count generated by RNA-seq for each sample
 
Submission date Oct 25, 2023
Last update date Jun 16, 2024
Contact name Byung-Kwan Jeong
E-mail(s) goodididid@gmail.com
Organization name Asan Medical Center
Department Pathology
Street address 88, Olympic-ro 43-gil
City Songpa-Gu
State/province Seoul
ZIP/Postal code 05505
Country South Korea
 
Platform ID GPL24247
Series (2)
GSE246221 A Mouse Model for Metabolic Dysfunction-associated Steatotic Liver Disease and Hepatocellular Carcinoma [RNA-seq]
GSE246223 A Mouse Model for Metabolic Dysfunction-associated Steatotic Liver Disease and Hepatocellular Carcinoma
Relations
BioSample SAMN37984420
SRA SRX22224738

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA

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