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Sample GSM785648 Query DataSets for GSM785648
Status Public on Aug 25, 2011
Title SHED SP1-transfected 2
Sample type RNA
 
Source name SHED SP1-transfected
Organism Homo sapiens
Characteristics cell type: stem cells from human exfoliated decidiuous teeth
Treatment protocol Transfection was performed using FUGEN®HD (Roche) kit and the expression plasmids for SP1, TP53 and as control an empty vector without an insert.
Growth protocol Cell were grown in DMEM medium (PAA, Pasching, Austria) at 37°C in 5% CO2
Extracted molecule total RNA
Extraction protocol Total RNAs were extracted using the RNeasy Plus Mini Kit (Qiagen) and quality-controlled using the RNA 6000 Nano LabChip (Agilent Technologies, Santa Clara, CA, USA)
Label biotin
Label protocol Double-stranded cDNA was prepared from 5 μg of total cellular RNA (cDNA synthesis kit, Affymetrix Santa Clara, CA, USA), in vitro transcribed using an IVT labeling kit, and the cRNA product purified using a GeneChip Sample Cleanup Module (Affymetrix). All procedures according to the Affymetrix protocol.
 
Hybridization protocol Hybridization to Affymetrix oligonucleotide microarray Human Gene 1.1 ST array was carried out overnight with biotin-labeled cRNA in a hybridization oven 640
Scan protocol GeneTitan scanner
Description dental progenitor/stem cells
Gene expression of SHED after 48 hours of transfection with a SP1 expression vector
Data processing Affymetrix GeneChip Operating Software (GCOS) 1.4 and the Expression Console 1.1. Software.
For the creation of the summarized log-signal the robust multi-chip analysis (RAM) algorithm was used.
 
Submission date Aug 24, 2011
Last update date Sep 23, 2011
Contact name Christian Morsczeck
E-mail(s) christian.morsczeck@klinik.uni-regensburg.de
Organization name University of Regensburg
Street address Franz-Josef Strauss Allee 11
City Regensburg
ZIP/Postal code 93053
Country Germany
 
Platform ID GPL11532
Series (1)
GSE31628 Gene expression profiles of DFCs and SHED 48 hours after in vitro transfection with a TP53 plasmid, a SP1 plasmid, or an empty vector.

Data table header descriptions
ID_REF
VALUE log RMA

Data table
ID_REF VALUE
7896736 5.110786
7896738 2.685141
7896740 2.18569
7896742 6.542487
7896744 2.723991
7896746 7.916155
7896748 4.085961
7896750 2.340427
7896752 7.460583
7896754 3.020385
7896756 4.227785
7896759 5.146967
7896761 4.271681
7896779 5.656581
7896798 6.54334
7896817 8.056783
7896822 8.666927
7896859 3.054811
7896861 3.018453
7896863 4.014283

Total number of rows: 28829

Table truncated, full table size 475 Kbytes.




Supplementary file Size Download File type/resource
GSM785648.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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