NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM785647 Query DataSets for GSM785647
Status Public on Aug 25, 2011
Title SHED SP1-transfected 1
Sample type RNA
 
Source name SHED SP1-transfected
Organism Homo sapiens
Characteristics cell type: stem cells from human exfoliated decidiuous teeth
Treatment protocol Transfection was performed using FUGEN®HD (Roche) kit and the expression plasmids for SP1, TP53 and as control an empty vector without an insert.
Growth protocol Cell were grown in DMEM medium (PAA, Pasching, Austria) at 37°C in 5% CO2
Extracted molecule total RNA
Extraction protocol Total RNAs were extracted using the RNeasy Plus Mini Kit (Qiagen) and quality-controlled using the RNA 6000 Nano LabChip (Agilent Technologies, Santa Clara, CA, USA)
Label biotin
Label protocol Double-stranded cDNA was prepared from 5 μg of total cellular RNA (cDNA synthesis kit, Affymetrix Santa Clara, CA, USA), in vitro transcribed using an IVT labeling kit, and the cRNA product purified using a GeneChip Sample Cleanup Module (Affymetrix). All procedures according to the Affymetrix protocol.
 
Hybridization protocol Hybridization to Affymetrix oligonucleotide microarray Human Gene 1.1 ST array was carried out overnight with biotin-labeled cRNA in a hybridization oven 640
Scan protocol GeneTitan scanner
Description dental progenitor/stem cells
Gene expression of SHED after 48 hours of transfection with a SP1 expression vector
Data processing Affymetrix GeneChip Operating Software (GCOS) 1.4 and the Expression Console 1.1. Software.
For the creation of the summarized log-signal the robust multi-chip analysis (RAM) algorithm was used.
 
Submission date Aug 24, 2011
Last update date Sep 23, 2011
Contact name Christian Morsczeck
E-mail(s) christian.morsczeck@klinik.uni-regensburg.de
Organization name University of Regensburg
Street address Franz-Josef Strauss Allee 11
City Regensburg
ZIP/Postal code 93053
Country Germany
 
Platform ID GPL11532
Series (1)
GSE31628 Gene expression profiles of DFCs and SHED 48 hours after in vitro transfection with a TP53 plasmid, a SP1 plasmid, or an empty vector.

Data table header descriptions
ID_REF
VALUE log RMA

Data table
ID_REF VALUE
7896736 4.598466
7896738 2.61837
7896740 2.128818
7896742 6.709739
7896744 2.837955
7896746 7.948671
7896748 4.11404
7896750 2.344931
7896752 7.703192
7896754 3.392469
7896756 4.066921
7896759 5.014909
7896761 4.115515
7896779 5.5525
7896798 6.447718
7896817 8.148555
7896822 8.738772
7896859 3.099654
7896861 2.948137
7896863 3.737354

Total number of rows: 28829

Table truncated, full table size 475 Kbytes.




Supplementary file Size Download File type/resource
GSM785647.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap