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Sample GSM785642 Query DataSets for GSM785642
Status Public on Aug 25, 2011
Title dental follicle cells SP1-transfected 2
Sample type RNA
 
Source name dental follicle cells SP1-transfected
Organism Homo sapiens
Characteristics cell type: dental follicle cells
Treatment protocol Transfection was performed using FUGEN®HD (Roche) kit and the expression plasmids for SP1, TP53 and as control an empty vector without an insert.
Growth protocol Cell were grown in DMEM medium (PAA, Pasching, Austria) at 37°C in 5% CO2
Extracted molecule total RNA
Extraction protocol Total RNAs were extracted using the RNeasy Plus Mini Kit (Qiagen) and quality-controlled using the RNA 6000 Nano LabChip (Agilent Technologies, Santa Clara, CA, USA)
Label biotin
Label protocol Double-stranded cDNA was prepared from 5 μg of total cellular RNA (cDNA synthesis kit, Affymetrix Santa Clara, CA, USA), in vitro transcribed using an IVT labeling kit, and the cRNA product purified using a GeneChip Sample Cleanup Module (Affymetrix). All procedures according to the Affymetrix protocol.
 
Hybridization protocol Hybridization to Affymetrix oligonucleotide microarray Human Gene 1.1 ST array was carried out overnight with biotin-labeled cRNA in a hybridization oven 640
Scan protocol GeneTitan scanner
Description dental progenitor/stem cells
Gene expression of dental follicle cells after 48 hours of transfection with a SP1 expression vector
Data processing Affymetrix GeneChip Operating Software (GCOS) 1.4 and the Expression Console 1.1. Software.
For the creation of the summarized log-signal the robust multi-chip analysis (RAM) algorithm was used.
 
Submission date Aug 24, 2011
Last update date Sep 23, 2011
Contact name Christian Morsczeck
E-mail(s) christian.morsczeck@klinik.uni-regensburg.de
Organization name University of Regensburg
Street address Franz-Josef Strauss Allee 11
City Regensburg
ZIP/Postal code 93053
Country Germany
 
Platform ID GPL11532
Series (1)
GSE31628 Gene expression profiles of DFCs and SHED 48 hours after in vitro transfection with a TP53 plasmid, a SP1 plasmid, or an empty vector.

Data table header descriptions
ID_REF
VALUE log RMA

Data table
ID_REF VALUE
7896736 4.679794
7896738 2.706527
7896740 2.252764
7896742 6.215954
7896744 3.009592
7896746 7.611627
7896748 4.328558
7896750 2.469765
7896752 7.899608
7896754 2.8613
7896756 4.091947
7896759 5.426206
7896761 4.24668
7896779 5.479477
7896798 5.919693
7896817 6.591676
7896822 7.944369
7896859 2.999742
7896861 3.045609
7896863 3.992003

Total number of rows: 28829

Table truncated, full table size 475 Kbytes.




Supplementary file Size Download File type/resource
GSM785642.CEL.gz 4.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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