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Sample GSM785640 Query DataSets for GSM785640
Status Public on Aug 25, 2011
Title dental follicle cells TP53-transfected 2
Sample type RNA
 
Source name dental follicle cells TP53-transfected
Organism Homo sapiens
Characteristics cell type: dental follicle cells
Treatment protocol Transfection was performed using FUGEN®HD (Roche) kit and the expression plasmids for SP1, TP53 and as control an empty vector without an insert.
Growth protocol Cell were grown in DMEM medium (PAA, Pasching, Austria) at 37°C in 5% CO2
Extracted molecule total RNA
Extraction protocol Total RNAs were extracted using the RNeasy Plus Mini Kit (Qiagen) and quality-controlled using the RNA 6000 Nano LabChip (Agilent Technologies, Santa Clara, CA, USA)
Label biotin
Label protocol Double-stranded cDNA was prepared from 5 μg of total cellular RNA (cDNA synthesis kit, Affymetrix Santa Clara, CA, USA), in vitro transcribed using an IVT labeling kit, and the cRNA product purified using a GeneChip Sample Cleanup Module (Affymetrix). All procedures according to the Affymetrix protocol.
 
Hybridization protocol Hybridization to Affymetrix oligonucleotide microarray Human Gene 1.1 ST array was carried out overnight with biotin-labeled cRNA in a hybridization oven 640
Scan protocol GeneTitan scanner
Description dental progenitor/stem cells
Gene expression of dental follicle cells after 48 hours of transfection with a TP53 expression vector
Data processing Affymetrix GeneChip Operating Software (GCOS) 1.4 and the Expression Console 1.1. Software.
For the creation of the summarized log-signal the robust multi-chip analysis (RAM) algorithm was used.
 
Submission date Aug 24, 2011
Last update date Sep 23, 2011
Contact name Christian Morsczeck
E-mail(s) christian.morsczeck@klinik.uni-regensburg.de
Organization name University of Regensburg
Street address Franz-Josef Strauss Allee 11
City Regensburg
ZIP/Postal code 93053
Country Germany
 
Platform ID GPL11532
Series (1)
GSE31628 Gene expression profiles of DFCs and SHED 48 hours after in vitro transfection with a TP53 plasmid, a SP1 plasmid, or an empty vector.

Data table header descriptions
ID_REF
VALUE log RMA

Data table
ID_REF VALUE
7896736 4.112266
7896738 2.548438
7896740 2.088441
7896742 6.466986
7896744 2.736481
7896746 7.882455
7896748 4.073339
7896750 2.34727
7896752 7.425175
7896754 2.859325
7896756 4.381346
7896759 5.341679
7896761 4.162237
7896779 5.464052
7896798 5.908098
7896817 6.385505
7896822 7.737135
7896859 3.213987
7896861 3.153605
7896863 4.098525

Total number of rows: 28829

Table truncated, full table size 475 Kbytes.




Supplementary file Size Download File type/resource
GSM785640.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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