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Sample GSM785637 Query DataSets for GSM785637
Status Public on Aug 25, 2011
Title dental follicle cells control, empty vector 1
Sample type RNA
 
Source name dental follicle cells control
Organism Homo sapiens
Characteristics cell type: dental follicle cells
Treatment protocol Transfection was performed using FUGEN®HD (Roche) kit and the expression plasmids for SP1, TP53 and as control an empty vector without an insert.
Growth protocol Cell were grown in DMEM medium (PAA, Pasching, Austria) at 37°C in 5% CO2
Extracted molecule total RNA
Extraction protocol Total RNAs were extracted using the RNeasy Plus Mini Kit (Qiagen) and quality-controlled using the RNA 6000 Nano LabChip (Agilent Technologies, Santa Clara, CA, USA)
Label biotin
Label protocol Double-stranded cDNA was prepared from 5 μg of total cellular RNA (cDNA synthesis kit, Affymetrix Santa Clara, CA, USA), in vitro transcribed using an IVT labeling kit, and the cRNA product purified using a GeneChip Sample Cleanup Module (Affymetrix). All procedures according to the Affymetrix protocol.
 
Hybridization protocol Hybridization to Affymetrix oligonucleotide microarray Human Gene 1.1 ST array was carried out overnight with biotin-labeled cRNA in a hybridization oven 640
Scan protocol GeneTitan scanner
Description dental progenitor/stem cells
Gene expression of dental follicle cells after 48 hours of transfection with an empty vector
Data processing Affymetrix GeneChip Operating Software (GCOS) 1.4 and the Expression Console 1.1. Software.
For the creation of the summarized log-signal the robust multi-chip analysis (RAM) algorithm was used.
 
Submission date Aug 24, 2011
Last update date Sep 23, 2011
Contact name Christian Morsczeck
E-mail(s) christian.morsczeck@klinik.uni-regensburg.de
Organization name University of Regensburg
Street address Franz-Josef Strauss Allee 11
City Regensburg
ZIP/Postal code 93053
Country Germany
 
Platform ID GPL11532
Series (1)
GSE31628 Gene expression profiles of DFCs and SHED 48 hours after in vitro transfection with a TP53 plasmid, a SP1 plasmid, or an empty vector.

Data table header descriptions
ID_REF
VALUE log RMA

Data table
ID_REF VALUE
7896736 4.83735
7896738 2.670798
7896740 2.316646
7896742 6.037283
7896744 2.880457
7896746 7.653419
7896748 4.189855
7896750 2.263573
7896752 7.531379
7896754 3.181691
7896756 4.221643
7896759 5.111366
7896761 4.19137
7896779 5.600205
7896798 5.703318
7896817 6.21806
7896822 7.639761
7896859 2.719556
7896861 2.992051
7896863 3.953258

Total number of rows: 28829

Table truncated, full table size 475 Kbytes.




Supplementary file Size Download File type/resource
GSM785637.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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