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Sample GSM7842750 Query DataSets for GSM7842750
Status Public on Apr 24, 2024
Title Poly(I:C) + artLCMV-E7E6 treated tumor biological replicate 1 DAY1
Sample type SRA
 
Source name Tumor
Organism Mus musculus
Characteristics tissue: Tumor
tag: Poly(I:C) + artLCMV-E7E6 treated
strain: C57BL/6
genotype: ID8.OVA.Vegf/GFP
Treatment protocol Seven days later, mice were intravenously treated with 1×105 RCV FFU artLCMV-E7E6 and received intratumoral injections of 50 μg of poly(I:C) in the right-sided tumor lesion on days 7, 10, and 13.
Growth protocol Mice were subcutaneously injected with 1.5×105 TC-1/A9 cells in the right flank. Seven days later, tumor were treated.
Extracted molecule total RNA
Extraction protocol On day 8 and day 14, mice were euthanized, and RNA from tumors was subjected to RNAseq analysis
TruSeq Stranded mRNA preparation
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description d1E7PlyR2
NovaSeq6000 150PE (150 x 2bp) 80M total reads 12Gb/sample
Data processing FastQC for quality control
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm39 using STAR
Quantification using FeatureCounts
Assembly: mm39
Supplementary files format and content: tab-delimited text files include log2CPM values for each Sample in day1 and day7
 
Submission date Oct 16, 2023
Last update date Apr 24, 2024
Contact name Jose Gonzalez
E-mail(s) jgonzalezgom@unav.es
Organization name FIMA
Street address C/PIO XII s/n
City Pamplona
State/province Navarra
ZIP/Postal code 31008
Country Spain
 
Platform ID GPL24247
Series (1)
GSE245436 The efficacy of LCMV-based cancer vaccines is unleashed by intratumoral injections of polyI:C
Relations
BioSample SAMN37831887
SRA SRX22099974

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA

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