|
Status |
Public on Apr 24, 2024 |
Title |
artLCMV-E7E6 treated tumor biological replicate 3 DAY1 |
Sample type |
SRA |
|
|
Source name |
Tumor
|
Organism |
Mus musculus |
Characteristics |
tissue: Tumor tag: artLCMV-E7E6 treated strain: C57BL/6 genotype: ID8.OVA.Vegf/GFP
|
Treatment protocol |
Seven days later, mice were intravenously treated with 1×105 RCV FFU artLCMV-E7E6 and received intratumoral injections of 50 μg of poly(I:C) in the right-sided tumor lesion on days 7, 10, and 13.
|
Growth protocol |
Mice were subcutaneously injected with 1.5×105 TC-1/A9 cells in the right flank. Seven days later, tumor were treated.
|
Extracted molecule |
total RNA |
Extraction protocol |
On day 8 and day 14, mice were euthanized, and RNA from tumors was subjected to RNAseq analysis TruSeq Stranded mRNA preparation
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
d1E7R3 NovaSeq6000 150PE (150 x 2bp) 80M total reads 12Gb/sample
|
Data processing |
FastQC for quality control Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm39 using STAR Quantification using FeatureCounts Assembly: mm39 Supplementary files format and content: tab-delimited text files include log2CPM values for each Sample in day1 and day7
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|
|
Submission date |
Oct 16, 2023 |
Last update date |
Apr 24, 2024 |
Contact name |
Jose Gonzalez |
E-mail(s) |
jgonzalezgom@unav.es
|
Organization name |
FIMA
|
Street address |
C/PIO XII s/n
|
City |
Pamplona |
State/province |
Navarra |
ZIP/Postal code |
31008 |
Country |
Spain |
|
|
Platform ID |
GPL24247 |
Series (1) |
GSE245436 |
The efficacy of LCMV-based cancer vaccines is unleashed by intratumoral injections of polyI:C |
|
Relations |
BioSample |
SAMN37831890 |
SRA |
SRX22099971 |