|
| Status |
Public on Oct 13, 2011 |
| Title |
posterior trunk expression replicate2 |
| Sample type |
RNA |
| |
|
| Source name |
Posterior trunk cells
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6CBAF1/J
age: E10.5
cell type: posterior trunk cells
|
| Treatment protocol |
Tissue samples were isolated in PBS supplemented with 10% Fetal Calf Serum and made single cell by collagenase treatment and applying a cell-strainer cap (BD Biosciences). Cells were takenb up in TrizolLS and stored at -80 degrees celcius.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TrizolLS extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
500 ng of total RNA was amplified and labeled using MessageAmp™ Premier RNA Amplification Kit (Ambion, AM1792). cRNA quality was assessed by capillary electrophoresis on an Agilent 2100 Bioanalyzer.
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affyemtrix GeneChip Scanner 3000 7G.
|
| Description |
Gene expression data from mouse E10.5 embryonic stage posterior trunk cells.
|
| Data processing |
The data were analyzed with GC-RMA (Bioconductor).
|
| |
|
| Submission date |
Aug 22, 2011 |
| Last update date |
Oct 13, 2011 |
| Contact name |
Marion LELEU |
| Organization name |
EPFL
|
| Department |
School of Life Sciences
|
| Lab |
Laboratory of developmental genomics
|
| Street address |
EPFL-SV-ISREC-UPDUB- Station 19
|
| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE31570 |
The Dynamic Architecture of Hox Gene Clusters |
|
