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Sample GSM783830 Query DataSets for GSM783830
Status Public on Oct 13, 2011
Title posterior trunk expression replicate2
Sample type RNA
Source name Posterior trunk cells
Organism Mus musculus
Characteristics strain: B6CBAF1/J
age: E10.5
cell type: posterior trunk cells
Treatment protocol Tissue samples were isolated in PBS supplemented with 10% Fetal Calf Serum and made single cell by collagenase treatment and applying a cell-strainer cap (BD Biosciences). Cells were takenb up in TrizolLS and stored at -80 degrees celcius.
Extracted molecule total RNA
Extraction protocol TrizolLS extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol 500 ng of total RNA was amplified and labeled using MessageAmp™ Premier RNA Amplification Kit (Ambion, AM1792). cRNA quality was assessed by capillary electrophoresis on an Agilent 2100 Bioanalyzer.
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix GeneChip Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affyemtrix GeneChip Scanner 3000 7G.
Description Gene expression data from mouse E10.5 embryonic stage posterior trunk cells.
Data processing The data were analyzed with GC-RMA (Bioconductor).
Submission date Aug 22, 2011
Last update date Oct 13, 2011
Contact name Marion LELEU
Organization name EPFL
Department School of Life Sciences
Lab Laboratory of developmental genomics
Street address EPFL-SV-ISREC-UPDUB- Station 19
City Lausanne
ZIP/Postal code 1015
Country Switzerland
Platform ID GPL1261
Series (1)
GSE31570 The Dynamic Architecture of Hox Gene Clusters

Data table header descriptions
VALUE log2 GC-RMA signal

Data table
1415670_at 10.49475
1415671_at 11.3856
1415672_at 12.63109
1415673_at 9.160965
1415674_a_at 10.75798
1415675_at 10.02244
1415676_a_at 12.29005
1415677_at 9.821906
1415678_at 11.11029
1415679_at 11.9193
1415680_at 10.46468
1415681_at 11.13402
1415682_at 10.16162
1415683_at 11.87108
1415684_at 9.813737
1415685_at 10.07686
1415686_at 10.44276
1415687_a_at 11.0685
1415688_at 12.08788
1415689_s_at 10.90532

Total number of rows: 45101

Table truncated, full table size 894 Kbytes.

Supplementary file Size Download File type/resource
GSM783830_posteriortrunk_rep2.CEL.gz 3.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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