|
| Status |
Public on Nov 05, 2023 |
| Title |
J558 allograft, Vehicle-1, Day 6 |
| Sample type |
SRA |
| |
|
| Source name |
allograft tumor
|
| Organism |
Mus musculus |
| Characteristics |
tissue: allograft tumor
cell line: J558
cell type: B lymphocyte
time: Day 6
treatment: 0.5% methylcellulose
|
| Treatment protocol |
The J558-allografted BALB/c female mice were orally administered 0.5% methylcellulose as vehicle or TAK-931 at 60 mg/kg, QD for 6 days.
|
| Growth protocol |
J558 cells were maintained in DMEM supplemented with 10% Horse Serum and penicillin/Streptomycin in humidified atmosphere with 5% CO2 at 37C. The female BALB/c mice were inoculated subcutaneously with 5 × 10e5 J558 cells each (in 0.1 mL, no Matrigel) in the right flank.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Rneasy Mini kit (QIAGEN).
RNA-seq was performed by Filgen, Inc.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Sequence read was mapped to mouse genome using STAR (v2.6.1c).
acquire only unique reads (map255)
Assembly: mm10
Supplementary files format and content: tab-delimited text file including expression level for each sample (featureCounts)
|
| |
|
| Submission date |
Oct 10, 2023 |
| Last update date |
Nov 05, 2023 |
| Contact name |
Akihiro Ohashi |
| E-mail(s) |
aohashi@east.ncc.go.jp
|
| Phone |
+81-4-7133-1111
|
| Organization name |
National Cancer Center Japan
|
| Department |
Exploratory Oncology Research and Clinical Center
|
| Lab |
Division of Collaborative Research and Development
|
| Street address |
6-5-1 Kashiwanoha
|
| City |
Kashiwa |
| State/province |
Chiba |
| ZIP/Postal code |
2778577 |
| Country |
Japan |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE244988 |
Transcriptome analysis of J558 allograft mouse model treated with the CDC7-specific inhibitor TAK-931 [RNA-seq] |
|
| Relations |
| BioSample |
SAMN37754216 |
| SRA |
SRX22048365 |
