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Sample GSM782668 Query DataSets for GSM782668
Status Public on Feb 13, 2012
Title RNA-seq of IL-10 treated macrophages
Sample type SRA
Source name RNA-seq of IL-10 treated macrophages
Organism Mus musculus
Characteristics strain: C57BL/6JJcl
gender: Male
cell type: Adherent peritoneal exudate macrophages
Treatment protocol Macrophages were treated with 100 ng/ml of IL-10 for four hours. Cells were then harvested with TRIzol and RNA extracted
Growth protocol peritoneal exudate cells were washed from the peritoneum 3 days after (.i.p.) thioglycolate injection. Cells were allowed to adhere for 24 hours and non-adherent cells were washed away with several washes of fresh medium. PEC macrophages where treated with IL-10 (100 ng/ml) for 4 hours.
Extracted molecule total RNA
Extraction protocol Library and Sequencing were performed at BGI, China, using standard Illumnina kits and protocols.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
Description library insert size: 188
RNA-seq of IL-10 treated macrophages
Data processing Alignment: Sequence reads were aligned to the mm9 mouse genome assembly using the TopHat and Cufflinks combination
Splice Junctions: A bed file containing the splice junctions as determined by the TopHat and Cufflinks combination
Submission date Aug 19, 2011
Last update date May 15, 2019
Contact name Andrew P Hutchins
Organization name Southern University of Science and Technology
Department Bioinformatics
Lab Bioinformatics and Genomics
Street address 1088 Xueyuan Rd
City Shenzhen
ZIP/Postal code 518055
Country China
Platform ID GPL13112
Series (2)
GSE31530 Transcriptome changes in IL-10 treated peritoneal macrophages
GSE31531 Genome-wide analysis of STAT3-binding and gene expression changes in peritoneal macrophages upon interleukin-10 stimulation
Reanalyzed by GSM1335242
SRA SRX092400
BioSample SAMN00710344

Supplementary file Size Download File type/resource
GSM782668_PEC_IL10-RNAseq-aligned.sam.gz 1.4 Gb (ftp)(http) SAM
GSM782668_PEC_IL10-RNAseq-junctions.bed.gz 2.2 Mb (ftp)(http) BED
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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