NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM7823146 Query DataSets for GSM7823146
Status Public on Oct 04, 2024
Title Platelets from NSCLC patient pre-treatment_P13F0
Sample type RNA
 
Source name blood platelet in tumour presence
Organism Homo sapiens
Characteristics blood: platelet
condition: tumour presence
histology: Lung adenocarcinoma
tnm: T1bcN0M0
Sex: Female
age: 58
Treatment protocol not applicable
Growth protocol not applicable
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using Leucocyte kit (Norgen, Canada) as per manufacturer's instructions
Label biotin
Label protocol Single-stranded cDNA was generated from the amplified cRNA with the WT Plus cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
 
Hybridization protocol Whole-genome gene expression profiling was performed using the Clariom S human gene expression array (Affymetrix) with Hybridization, Wash, and Stain Kit (Affymetrix) following manufacturer's instructions
Scan protocol Clariom S chips were scanned with the GeneChip Scanner 3000 7G (Affymetrix)
Data processing Raw data was imported to Affymetrix software Transcriptome Analysis Console (TAC) and quality control (QC) was performed as manufacturer's instructions (PCA, labeling controls, hybridization controls, Pos vs. Neg AUC, and Signal Box Plot). Normalization was performed with the Robust Multi-chip Analysis (RMA) approach with default analysis settings.
 
Submission date Oct 04, 2023
Last update date Oct 04, 2024
Contact name Patricia Severino
E-mail(s) patricia.severino@einstein.br
Phone 5511984661282
Organization name Hospital Israelita Albert Einstein
Department Albert Einstein Research and Education Institute
Street address Rua Comendador Elias Jafet, 755
City Sao Paulo
State/province Sao Paulo
ZIP/Postal code 05653-000
Country Brazil
 
Platform ID GPL23159
Series (2)
GSE244645 Expression data from platelets from early NSCLC and HNSCC patients in tumour presence and tumor free samples
GSE244647 NSCLC and HNSCC patients

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
TC0100006437.hg.1 3.41
TC0100006476.hg.1 5.54
TC0100006479.hg.1 5.75
TC0100006480.hg.1 4.79
TC0100006483.hg.1 3.88
TC0100006486.hg.1 4.55
TC0100006490.hg.1 3.33
TC0100006492.hg.1 5.14
TC0100006494.hg.1 4.24
TC0100006497.hg.1 3.82
TC0100006499.hg.1 5.4
TC0100006501.hg.1 4.45
TC0100006502.hg.1 3.84
TC0100006514.hg.1 4.39
TC0100006516.hg.1 4.98
TC0100006517.hg.1 3.53
TC0100006524.hg.1 4.67
TC0100006540.hg.1 3.8
TC0100006548.hg.1 3.49
TC0100006550.hg.1 4.7

Total number of rows: 21448

Table truncated, full table size 483 Kbytes.




Supplementary file Size Download File type/resource
GSM7823146_P13F0.CEL.gz 1.1 Mb (ftp)(http) CEL

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap