|
| Status |
Public on Mar 28, 2024 |
| Title |
GL261-GSCs-rep1 |
| Sample type |
SRA |
| |
|
| Source name |
Brain
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Brain
cell line: GL261-GSCs
cell type: Glioblastoma stem cell
|
| Growth protocol |
GL261 cells were grown in FCS-containing differentiation medium. GL261-GSCs were grown in defined GSC medium.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Cells and neurospheres were dissociated using Accutase and lysed in lysis buffer containing 0.3% IGEPAL® CA-630
Single cells or nuclei were captured in droplet emulsions using the Chromium instrument (10x Genomics) and scRNA-seq libraries were constructed as per the 10x Genomics protocol using GemCode Single-Cell 5’ Bead and Library kit.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Demultiplexing, barcode processing, gene counting and aggregation were made using Cell Ranger software
Assembly: mm10
Supplementary files format and content: 10xcells_processed.h5ad: annotated file in h5ad format
|
| |
|
| Submission date |
Sep 28, 2023 |
| Last update date |
Mar 28, 2024 |
| Contact name |
Arantxa Tabernero |
| E-mail(s) |
ataber@usal.es
|
| Phone |
34923294500
|
| Organization name |
Universidad de Salamanca
|
| Department |
INCyL
|
| Street address |
Pintor Fernando Gallego 1
|
| City |
Salamanca |
| ZIP/Postal code |
37007 |
| Country |
Spain |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE244301 |
Single-cell RNA-sequencing (scRNA-Seq) of GL261 and GL261-GSCs |
|
| Relations |
| BioSample |
SAMN37595475 |
| SRA |
SRX21923895 |
