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Sample GSM780878 Query DataSets for GSM780878
Status Public on Aug 18, 2011
Title MCF7 cells after 72hr treatment with 10uM 5-Aza-2'-deoxycytidine(Aza)
Sample type RNA
 
Channel 1
Source name treatment with 10uM 5-Aza-2'-deoxycytidine(Aza)
Organism Homo sapiens
Characteristics treatment: 10uM 5-Aza-2'-deoxycytidine for 72 h
cell line: MCF7
Treatment protocol A human breast cancer cell line, MCF-7, was treated with 5-Aza-2'-deoxycytidine(Aza), at 5μM and 10μM for 72hr. Culture medium with or without treatment was changed every 24 hr.
Growth protocol A human breast cancer cell line, MCF-7, was grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS).
Extracted molecule total RNA
Extraction protocol Total RNA from the MCF-7 cell was isolated using the Trizol (Qiagen, Valencia, CA) according to the supplier's protocols.
Label Cy3
Label protocol standard Phalanx protocol (See http://www.phalanxbiotech.com/)
 
Channel 2
Source name untreated
Organism Homo sapiens
Characteristics treatment: untreated
cell line: MCF7
Treatment protocol A human breast cancer cell line, MCF-7, was treated with 5-Aza-2'-deoxycytidine(Aza), at 5μM and 10μM for 72hr. Culture medium with or without treatment was changed every 24 hr.
Growth protocol A human breast cancer cell line, MCF-7, was grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS).
Extracted molecule total RNA
Extraction protocol Total RNA from the MCF-7 cell was isolated using the Trizol (Qiagen, Valencia, CA) according to the supplier's protocols.
Label Cy5
Label protocol standard Phalanx protocol (See http://www.phalanxbiotech.com/)
 
 
Hybridization protocol standard Phalanx protocol (See http://www.phalanxbiotech.com/)
Scan protocol standard Phalanx protocol (See http://www.phalanxbiotech.com/)
Description Analysis used aerobic steady state RNA as control samples for comparison to the experimental samples taken at 5, 10, 15 and 60 min.
Data processing standard Phalanx protocol (See http://www.phalanxbiotech.com/)
 
Submission date Aug 17, 2011
Last update date Aug 18, 2011
Contact name Ju Hee Kim
Organization name Dongguk University
Department Life Science
Lab Molecular Biology
Street address pil-dong 3-ga
City seoul
ZIP/Postal code 100-715
Country South Korea
 
Platform ID GPL6254
Series (1)
GSE31441 MCF7 cells gene expression change by inducing CpG demethylation using 5-Aza-2'-deoxycytidine (Aza)

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (experiment/control)

Data table
ID_REF VALUE
PH_hs_0000002 -1.311148256
PH_hs_0000003 -1.029146346
PH_hs_0000004 0.062121712
PH_hs_0000005 1.635522241
PH_hs_0000006 0.635986504
PH_hs_0000007 0.285698126
PH_hs_0000008 -0.454031631
PH_hs_0000009 0.301002256
PH_hs_0000010 -0.380821784
PH_hs_0000011 -0.305788392
PH_hs_0000012 -0.424687669
PH_hs_0000013 -0.109358756
PH_hs_0000014 0.386259141
PH_hs_0000015 -0.316528107
PH_hs_0000016 -0.307572802
PH_hs_0000017 -0.678071905
PH_hs_0000018 0.545968369
PH_hs_0000019 -0.652901329
PH_hs_0000020 -0.272297327
PH_hs_0000021 0.37517848

Total number of rows: 30968

Table truncated, full table size 756 Kbytes.




Supplementary file Size Download File type/resource
GSM780878_Phx0604044123_Ch1_Cy3_Aza.txt.gz 2.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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