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Sample GSM780877 Query DataSets for GSM780877
Status Public on Aug 18, 2011
Title MCF7 cells after 72hr treatment with 5uM 5-Aza-2'-deoxycytidine(Aza)
Sample type RNA
 
Channel 1
Source name treatment with 5uM 5-Aza-2'-deoxycytidine(Aza)
Organism Homo sapiens
Characteristics treatment: 5uM 5-Aza-2'-deoxycytidine for 72 h
cell line: MCF7
Treatment protocol A human breast cancer cell line, MCF-7, was treated with 5-Aza-2'-deoxycytidine(Aza), at 5μM and 10μM for 72hr. Culture medium with or without treatment was changed every 24 hr.
Growth protocol A human breast cancer cell line, MCF-7, was grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS).
Extracted molecule total RNA
Extraction protocol Total RNA from the MCF-7 cell was isolated using the Trizol (Qiagen, Valencia, CA) according to the supplier's protocols.
Label Cy3
Label protocol standard Phalanx protocol (See http://www.phalanxbiotech.com/)
 
Channel 2
Source name untreated
Organism Homo sapiens
Characteristics treatment: untreated
cell line: MCF7
Treatment protocol A human breast cancer cell line, MCF-7, was treated with 5-Aza-2'-deoxycytidine(Aza), at 5μM and 10μM for 72hr. Culture medium with or without treatment was changed every 24 hr.
Growth protocol A human breast cancer cell line, MCF-7, was grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS).
Extracted molecule total RNA
Extraction protocol Total RNA from the MCF-7 cell was isolated using the Trizol (Qiagen, Valencia, CA) according to the supplier's protocols.
Label Cy5
Label protocol standard Phalanx protocol (See http://www.phalanxbiotech.com/)
 
 
Hybridization protocol standard Phalanx protocol (See http://www.phalanxbiotech.com/)
Scan protocol standard Phalanx protocol (See http://www.phalanxbiotech.com/)
Description Analysis used aerobic steady state RNA as control samples for comparison to the experimental samples taken at 5, 10, 15 and 60 min.
Data processing standard Phalanx protocol (See http://www.phalanxbiotech.com/)
 
Submission date Aug 17, 2011
Last update date Aug 18, 2011
Contact name Ju Hee Kim
Organization name Dongguk University
Department Life Science
Lab Molecular Biology
Street address pil-dong 3-ga
City seoul
ZIP/Postal code 100-715
Country South Korea
 
Platform ID GPL6254
Series (1)
GSE31441 MCF7 cells gene expression change by inducing CpG demethylation using 5-Aza-2'-deoxycytidine (Aza)

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (experiment/control)

Data table
ID_REF VALUE
PH_hs_0000002 0.425459305
PH_hs_0000003 -0.051399153
PH_hs_0000004 0.336854639
PH_hs_0000005 0.155749233
PH_hs_0000006 1.102993993
PH_hs_0000007 -0.103146927
PH_hs_0000008 -0.182786076
PH_hs_0000009 -0.224317298
PH_hs_0000010 -0.177881725
PH_hs_0000011 0.176322773
PH_hs_0000012 -0.307572802
PH_hs_0000013 -0.477944251
PH_hs_0000014 0.255802837
PH_hs_0000015 0.270229907
PH_hs_0000016 -0.089267338
PH_hs_0000017 0.729444007
PH_hs_0000018 0.011495639
PH_hs_0000019 -0.126580497
PH_hs_0000020 0.164786278
PH_hs_0000021 -0.147202107

Total number of rows: 30968

Table truncated, full table size 781 Kbytes.




Supplementary file Size Download File type/resource
GSM780877_Phx0604044268_Ch1_Cy3_Aza.txt.gz 2.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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