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Sample GSM7792033 Query DataSets for GSM7792033
Status Public on Jan 25, 2024
Title RNAseq_ZSCAN4_KO_Ctrl_rep1
Sample type SRA
 
Source name Embryonic cells
Organism Homo sapiens
Characteristics tissue: Embryonic cells
cell line: ES cells (SEES3)
cell type: Pluripotent stem cells
genotype: Male
treatment: Ctrl
replication: Biological replicate 1
Extracted molecule polyA RNA
Extraction protocol Total RNA was extracted using TRIzol solution (Roche) according to the manufacturer's instructions
The cDNA library was prepared from 500ng of each total RNA sample for massive parallel sequencing using an NEBNext Poly(A) mRNA Magnetic Isolation Module and an Ultra Directional RNA Library Prep Kit for Illumina (NEB). The cDNA library produced ranged from 400 to 1000 bp, including the adaptor sequences
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Data processing Fastq files were processed and analyzed on the Biowardrobe platform
Heat maps were generated by Morpheus
Assembly: hg19
Supplementary files format and content: tab-delimited text files include RPKM values for each Sample
 
Submission date Sep 20, 2023
Last update date Jan 25, 2024
Contact name Tomohiko Akiyama
E-mail(s) akiakiakit@hotmail.com
Phone 0457872596
Organization name Yokohama city university
Department School of medicine
Street address 3-9 Fuku-ura
City Yokohama
State/province Kanagawa
ZIP/Postal code 236-0004
Country Japan
 
Platform ID GPL16791
Series (2)
GSE243627 ZSCAN4-binding motif - TGCACAC is conserved and enriched in CA/TG microsatellites in both mouse and human genomes [RNA-Seq]
GSE243628 ZSCAN4-binding motif - TGCACAC is conserved and enriched in CA/TG microsatellites in both mouse and human genomes
Relations
BioSample SAMN37479654
SRA SRX21832896

Supplementary file Size Download File type/resource
GSM7792033_RNAseq_ZSCAN4_KO_Ctrl_rep1.txt.gz 545.7 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA

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