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Sample GSM7791913 Query DataSets for GSM7791913
Status Public on Dec 08, 2023
Title Amor_L1_uPAR_positive
Sample type SRA
 
Source name Liver
Organism Mus musculus
Characteristics tissue: Liver
genotype: WT
strain: C57BL6/J
treatment: Untreated
Sex: Female
Extracted molecule total RNA
Extraction protocol Live uPAR positive or negative cells were sorted by uPAR staining (R&D, FAB531P) on a SONY SH800 cell sorter, stained with AO/PI (acridine orange/propidium iodide, Nexcelon ViaStain) and checked for viability >80% using a Countess FL II Automated cell counter.
10X Genomics NextGEM v3.1 Single Cell 3' Gene Expression libraries were prepared from sorted cells according to the manufacturer's instructions and sequenced on Illumina NextSeq2000 flow cells.
 
Library strategy RNA-Seq
Library source transcriptomic single cell
Library selection cDNA
Instrument model Illumina NextSeq 500
 
Description 10x Genomics
Data processing Sequencing data was demultiplexed, mapped, and processed into gene/cell expression matrices using 10X Genomics' Cell Ranger software v7.1.0 (https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger). Gene expression reads were aligned to the mouse reference genome version gex-mm10-2020-A, available from the 10X Genomics website.
Assembly: mm10
Supplementary files format and content: Filtered single cell 3' cell/gene matrices in .h5 format generated by Cell Ranger for Liver + Adipose tissues
Supplementary files format and content: Filtered single cell 3' cell/gene matrices in .h5 format generated by Cell Ranger for Pancreas tissue
 
Submission date Sep 20, 2023
Last update date Dec 08, 2023
Contact name Yu-Jui Ho
E-mail(s) hoy@mskcc.org
Organization name Memorial Sloan Kettering Cancer Center
Department Cancer Biology & Genetics Program
Street address 417 E 68th St
City New York
State/province New York
ZIP/Postal code 10065
Country USA
 
Platform ID GPL19057
Series (1)
GSE243616 Prophilactic and long-lasting efficacy of senolytic CAR T cells against age-related metabolic dysfunction.
Relations
BioSample SAMN37475391
SRA SRX21831072

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA

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