NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM7783608 Query DataSets for GSM7783608
Status Public on Nov 12, 2024
Title Basal cell P13, Duct 2
Sample type SRA
 
Source name prostate
Organism Mus musculus
Characteristics tissue: prostate
cell type: tip, ventral lobe
age: P10-12
Sex: Male
Extracted molecule total RNA
Extraction protocol RNA extraction from FACS isolated cells was performed using RNeasy micro kit (QIAGEN) according to the manufacturer ́s recommendations.
Indexed cDNA libraries were obtained using the Ovation Solo RNA-seq Systems (NuGen) following manufacturer’s recommendations.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description Elsa4-B13D2.4
Data processing Reads were mapped against the mouse reference genome (Grcm38/mm10) using STAR software to generate read alignments for each sample.
Annotations for Mus_musculus.GRCm38.87.gtf were obtained from ftp.Ensembl.org.
After transcripts assembling, gene level counts were obtained using HTseq and normalized to 20 millions of aligned reads.
Assembly: mm10/Grcm38.87
Supplementary files format and content: .csv / normalised gene expression
 
Submission date Sep 15, 2023
Last update date Nov 12, 2024
Contact name Yura Song
E-mail(s) yura.song@ulb.be
Organization name Université Libre de Bruxelles
Lab Laboratory of Stem Cells and Cancer
Street address 808, route de Lennik
City Bruxelles
ZIP/Postal code 1070
Country Belgium
 
Platform ID GPL24247
Series (2)
GSE243336 Collagen signaling and matrix stiffness regulate multipotencyin glandular epithelial stem cells in mice [bulk RNA-Seq]
GSE243338 Collagen signaling and matrix stiffness regulate multipotency in glandular epithelial stem cells in mice
Relations
BioSample SAMN37411422
SRA SRX21787300

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap