|
Status |
Public on Nov 12, 2024 |
Title |
Basal cell P13, Duct 2 |
Sample type |
SRA |
|
|
Source name |
prostate
|
Organism |
Mus musculus |
Characteristics |
tissue: prostate cell type: tip, ventral lobe age: P10-12 Sex: Male
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA extraction from FACS isolated cells was performed using RNeasy micro kit (QIAGEN) according to the manufacturer ́s recommendations. Indexed cDNA libraries were obtained using the Ovation Solo RNA-seq Systems (NuGen) following manufacturer’s recommendations.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
Elsa4-B13D2.4
|
Data processing |
Reads were mapped against the mouse reference genome (Grcm38/mm10) using STAR software to generate read alignments for each sample. Annotations for Mus_musculus.GRCm38.87.gtf were obtained from ftp.Ensembl.org. After transcripts assembling, gene level counts were obtained using HTseq and normalized to 20 millions of aligned reads. Assembly: mm10/Grcm38.87 Supplementary files format and content: .csv / normalised gene expression
|
|
|
Submission date |
Sep 15, 2023 |
Last update date |
Nov 12, 2024 |
Contact name |
Yura Song |
E-mail(s) |
yura.song@ulb.be
|
Organization name |
Université Libre de Bruxelles
|
Lab |
Laboratory of Stem Cells and Cancer
|
Street address |
808, route de Lennik
|
City |
Bruxelles |
ZIP/Postal code |
1070 |
Country |
Belgium |
|
|
Platform ID |
GPL24247 |
Series (2) |
GSE243336 |
Collagen signaling and matrix stiffness regulate multipotencyin glandular epithelial stem cells in mice [bulk RNA-Seq] |
GSE243338 |
Collagen signaling and matrix stiffness regulate multipotency in glandular epithelial stem cells in mice |
|
Relations |
BioSample |
SAMN37411422 |
SRA |
SRX21787300 |