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Sample GSM7778669 Query DataSets for GSM7778669
Status Public on Jan 11, 2024
Title DRG 3
Sample type SRA
 
Source name DRG
Organism Homo sapiens
Characteristics tissue: DRG
age: 50 years old
Extracted molecule polyA RNA
Extraction protocol Samples were immediately cleaned from the blood and connective tissue and frozen on dry ice.The whole ganglia were embedded in OCT for spatial transcriptomics.Cryosections were cut at 10-μm thickness and mounted onto the GEX arrays. Sections were placed on a Thermocycler Adaptor with the active surface facing up and were incubated at 37°C for 1 min. Then, sections were fixed with methyl alcohol at -20°C for 30 min, followed by staining with hematoxylin and eosin (H&E) (Eosin, Dako CS701, Hematoxylin Dako S3309, bluing buffer CS702).
Visum spatial libraries were constructed using Visum spatial Library construction kit (10x Genomics, PN-1000184) according to the manufacture’s introduction.
 
Library strategy OTHER
Library source transcriptomic
Library selection other
Instrument model Illumina NovaSeq 6000
 
Data processing Basecalls performed using bcl2fastq tools
Visum spatial gene expression was processed using Visum spatial gene expression slide and Reagent Kit (10x Genomics, PN-1000184).
Assembly: GRCh38
Supplementary files format and content: Tab separated values files with barcode sequences corresponding to column indices, features corresponding to row indices
Library strategy: spatial transcriptomics
 
Submission date Sep 13, 2023
Last update date Jan 11, 2024
Contact name Donghang Zhang
Organization name West China Hospital of Sichuan University
Street address No.37, guoxue lane, wuhou district, chengdu city, sichuan province
City Chengdu
State/province Sichuan
ZIP/Postal code 610041
Country China
 
Platform ID GPL24676
Series (2)
GSE243073 Spatial transcriptomics and single-nucleus RNA-sequencing reveal a transcriptomic atlas of human spinal cord [DRG Spatial]
GSE243077 Spatial transcriptomics and single-nucleus RNA-sequencing reveal a transcriptomic atlas of human spinal cord
Relations
BioSample SAMN37381922
SRA SRX21766805

Supplementary file Size Download File type/resource
GSM7778669_DRG3_filtered_feature_bc_matrix.tar.gz 10.0 Mb (ftp)(http) TAR
GSM7778669_DRG_3_ch00.jpg.gz 32.8 Mb (ftp)(http) JPG
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Raw data are available in SRA

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