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Sample GSM7766606 Query DataSets for GSM7766606
Status Public on Sep 27, 2024
Title Otof_het_Rep1
Sample type SRA
 
Source name cochlea
Organism Mus musculus
Characteristics tissue: cochlea
genotype: Heterozygous
treatment: PostnatalDay7
Extracted molecule total RNA
Extraction protocol cochlear tissues were homogenized with Qiagen's RLT buffer using a plastic homogenizer and RNA eas extracted using the Qiagen microRNAeasy column. Samples were tested for a RIN values >9 before sequencing.
mRNA was purfied using poly-T olgio attached magentic beads. After fragmentation, the frist strand cDNA was synthesized usign random hexamer primers, followed by second stand cDNA synthesis using dTTP primers. End repari, A-tailing was reported, before adapter ligation and size seldction.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description Het_Rep1
Data processing NextFlow RNA pipeline
Salmon used for counting transcripts.
DeSeq2 used for differential expression analysis.
Assembly: mm10
Supplementary files format and content: Otof_salmon_genecounts.tsv; raw gene counts from salmon used as input for DESEQ2
Supplementary files format and content: DEGsOtofKOP7.xlsx; differentially expressed genes from Deseq2
 
Submission date Sep 07, 2023
Last update date Sep 27, 2024
Contact name Fiorella Grandi
E-mail(s) fiorella.grandi0@gmail.com
Organization name Sorbonne Universite
Department Centre de Recherche de Myologie
Lab UMRS 974
Street address 105 Bld d'Hopital
City Paris
ZIP/Postal code 75013
Country France
 
Platform ID GPL24247
Series (1)
GSE242669 The upregulation of K+ and HCN channels in developing spiral ganglion neurons is mediated by cochlear inner hair cells
Relations
BioSample SAMN37316922
SRA SRX21663311

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA

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