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Sample GSM7763800 Query DataSets for GSM7763800
Status Public on Jan 01, 2024
Sample type SRA
Source name scraped urothelium
Organism Mus musculus
Characteristics tissue: scraped urothelium
strain: C57BL/6
Extracted molecule total RNA
Extraction protocol Single cell suspensions were generated using the Miltenyi Biotec Tumor Dissociation Kit and the gentleMACS Dissociator. Red blood cells were lysed using Miltenyi Biotec Red Blood Cell Lysis Solution and dead cells were removed using the Miltenyi Biotec Dead Cell Removal Kit. Single cell suspensions were subsequently loaded onto the 10X Genomics Chromium Controller to generate single-cell gel beads in emulsions (GEMs)
scRNAseq libraries were prepared using Chromium Single Cell 3' Reagent Kits (v3.1 Chemistry) according to the manufacter's instructions
Library strategy RNA-Seq
Library source transcriptomic single cell
Library selection cDNA
Instrument model Illumina NovaSeq 6000
Description 10x Genomics
Data processing The demultiplexing, barcoded processing, gene counting were made using the Cell Ranger software v6.1.1
Assembly: mm10
Supplementary files format and content: H5 format files of cell ranger output for each scRNAseq samples
Submission date Sep 06, 2023
Last update date Jan 01, 2024
Contact name William Kim
Organization name UNC Chapel Hill
Department Lineberger Comprehensive Cancer Center
Street address 450 West Dr
City Chapel Hill
State/province NC
ZIP/Postal code 27599
Country USA
Platform ID GPL24247
Series (1)
GSE217093 RNAseq and scRNAseq analysis of UPFL mouse tumors
BioSample SAMN37300245
SRA SRX21646651

Supplementary file Size Download File type/resource
GSM7763800_WYKNIU2.filtered_feature_bc_matrix.h5 29.9 Mb (ftp)(http) H5
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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