|
|
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Aug 06, 2011 |
Title |
MOLF backcross animal 4030 |
Sample type |
SRA |
|
|
Source name |
PyMT-induced mammary tumor
|
Organism |
Mus musculus |
Characteristics |
strain background: ((MOLF x PyMT) x FVB) backcross animal tissue: MOLF x PyMT backcross mammary tumor
|
Extracted molecule |
total RNA |
Extraction protocol |
Illumina mRNA Library Preparation Protocol (Total RNA extracted from mammary tissue, Purify mRNA by poly-A selection, Fragment mRNA, Synthesize the First Strand cDNA, Synthesize the Second Strand cDNA, Perform End Repair, Adenylate 3' ends, Ligate the Adapters, Purify the cDNA Templates on a gel to select a size range)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2000 |
|
|
Description |
mean insert size : 242.5
|
Data processing |
Illumina software pipeline ( Basecalls was done by using Illumina RTA 1.6 for GAIIx run, RTA 1.7 for HiSeq run , Alignment was done by using Illumina CASAVA1.6, CASAVA_v1.7.0 pipelines)
|
|
|
Submission date |
Aug 04, 2011 |
Last update date |
May 15, 2019 |
Contact name |
Ying Hu |
E-mail(s) |
yhu@mail.nih.gov, hunterk@mail.nih.gov
|
Phone |
240 276 5273
|
Organization name |
NIH
|
Department |
NCI/CCR
|
Lab |
CBIIT
|
Street address |
9609 Medical Center Dr.
|
City |
Rockville |
State/province |
MD |
ZIP/Postal code |
20850 |
Country |
USA |
|
|
Platform ID |
GPL13112 |
Series (2) |
GSE30866 |
Gene expression of polyoma middle T antigen induced mammary tumors |
GSE31223 |
Gene expression of polyoma middle T antigen induced mammary tumors [RNA_Seq : MOLF x PyMT] |
|
Relations |
SRA |
SRX093385 |
BioSample |
SAMN00714260 |
Supplementary file |
Size |
Download |
File type/resource |
GSM774019_NCIMouse4030_Gene_RPKM.txt.gz |
255.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
Processed data are available on Series record |
|
|
|
|
|