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Sample GSM774018 Query DataSets for GSM774018
Status Public on Aug 06, 2011
Title MOLF backcross animal 4020
Sample type SRA
Source name PyMT-induced mammary tumor
Organism Mus musculus
Characteristics strain background: ((MOLF x PyMT) x FVB) backcross animal
tissue: MOLF x PyMT backcross mammary tumor
Extracted molecule total RNA
Extraction protocol Illumina mRNA Library Preparation Protocol (Total RNA extracted from mammary tissue, Purify mRNA by poly-A selection, Fragment mRNA, Synthesize the First Strand cDNA, Synthesize the Second Strand cDNA, Perform End Repair, Adenylate 3' ends, Ligate the Adapters, Purify the cDNA Templates on a gel to select a size range)
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
Description mean insert size : 230.6
Data processing Illumina software pipeline ( Basecalls was done by using Illumina RTA 1.6 for GAIIx run, RTA 1.7 for HiSeq run , Alignment was done by using Illumina CASAVA1.6, CASAVA_v1.7.0 pipelines)
Submission date Aug 04, 2011
Last update date May 15, 2019
Contact name Ying Hu
Phone 240 276 5273
Organization name NIH
Department NCI/CCR
Street address 9609 Medical Center Dr.
City Rockville
State/province MD
ZIP/Postal code 20850
Country USA
Platform ID GPL13112
Series (2)
GSE30866 Gene expression of polyoma middle T antigen induced mammary tumors
GSE31223 Gene expression of polyoma middle T antigen induced mammary tumors [RNA_Seq : MOLF x PyMT]
SRA SRX093384
BioSample SAMN00714259

Supplementary file Size Download File type/resource
GSM774018_NCIMouse4020_Gene_RPKM.txt.gz 255.8 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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