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Sample GSM774016 Query DataSets for GSM774016
Status Public on Aug 06, 2011
Title MOLF backcross animal 3939
Sample type SRA
 
Source name PyMT-induced mammary tumor
Organism Mus musculus
Characteristics strain background: ((MOLF x PyMT) x FVB) backcross animal
tissue: MOLF x PyMT backcross mammary tumor
Extracted molecule total RNA
Extraction protocol Illumina mRNA Library Preparation Protocol (Total RNA extracted from mammary tissue, Purify mRNA by poly-A selection, Fragment mRNA, Synthesize the First Strand cDNA, Synthesize the Second Strand cDNA, Perform End Repair, Adenylate 3' ends, Ligate the Adapters, Purify the cDNA Templates on a gel to select a size range)
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina Genome Analyzer IIx
 
Description mean insert size : 248.6
Data processing Illumina software pipeline ( Basecalls was done by using Illumina RTA 1.6 for GAIIx run, RTA 1.7 for HiSeq run , Alignment was done by using Illumina CASAVA1.6, CASAVA_v1.7.0 pipelines)
 
Submission date Aug 04, 2011
Last update date May 15, 2019
Contact name Ying Hu
E-mail(s) yhu@mail.nih.gov, hunterk@mail.nih.gov
Phone 240 276 5273
Organization name NIH
Department NCI/CCR
Lab CBIIT
Street address 9609 Medical Center Dr.
City Rockville
State/province MD
ZIP/Postal code 20850
Country USA
 
Platform ID GPL11002
Series (2)
GSE30866 Gene expression of polyoma middle T antigen induced mammary tumors
GSE31223 Gene expression of polyoma middle T antigen induced mammary tumors [RNA_Seq : MOLF x PyMT]
Relations
SRA SRX093382
BioSample SAMN00714257

Supplementary file Size Download File type/resource
GSM774016_NCIMouse3939_Gene_RPKM.txt.gz 254.7 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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