|
Status |
Public on Sep 01, 2024 |
Title |
normoxic control, wild type, replicate 3 |
Sample type |
SRA |
|
|
Source name |
whole fly
|
Organism |
Drosophila melanogaster |
Characteristics |
tissue: whole fly genotype: het control (12/+) treatment: normox replicate: 3
|
Treatment protocol |
Flies were kept in a hypoxic environment of 1% oxygen for 12 hours before being allowed to recover for 1 or 12 hours in a normoxic environment
|
Growth protocol |
Flies were maintained at 25°C, 60% humidity, 12h/12h light/dark cycle, on Bloomington food, except during hypoxia treatment
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Flies were homogenized in trizol + ceramic beads Illumina Stranded mRNA prep, 96 index Poly-A-selected total RNA prepared with TruSeq ligation for paired-end sequencing
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
NextSeq 550 |
|
|
Data processing |
QC with FastQC and MultiQC Quantification with salmon Quantifications imported with tximport Normalization and transformed log counts with DESeq2 Assembly: BDGP6.32 Supplementary files format and content: salmon quantification directories as gzip'd archives Supplementary files format and content: supplementary file contained transformed log counts of all samples
|
|
|
Submission date |
Aug 24, 2023 |
Last update date |
Sep 01, 2024 |
Contact name |
Jason M Tennessen |
E-mail(s) |
tennessenlab@gmail.com, tennlab@iu.edu, jtenness@iu.edu, tourigny@iu.edu
|
Phone |
8128559803
|
Organization name |
Indiana University
|
Department |
Biology
|
Lab |
Tennessen
|
Street address |
1001 E 3rd St
|
City |
Bloomington |
State/province |
IN |
ZIP/Postal code |
47401 |
Country |
USA |
|
|
Platform ID |
GPL22106 |
Series (1) |
GSE241662 |
Transcriptomics of impaired hypoxic recovery of L2HGDH mutants |
|
Relations |
BioSample |
SAMN37145617 |
SRA |
SRX21480231 |