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Sample GSM7732864 Query DataSets for GSM7732864
Status Public on Sep 01, 2024
Title normoxic control, wild type, replicate 3
Sample type SRA
 
Source name whole fly
Organism Drosophila melanogaster
Characteristics tissue: whole fly
genotype: het control (12/+)
treatment: normox
replicate: 3
Treatment protocol Flies were kept in a hypoxic environment of 1% oxygen for 12 hours before being allowed to recover for 1 or 12 hours in a normoxic environment
Growth protocol Flies were maintained at 25°C, 60% humidity, 12h/12h light/dark cycle, on Bloomington food, except during hypoxia treatment
Extracted molecule polyA RNA
Extraction protocol Flies were homogenized in trizol + ceramic beads
Illumina Stranded mRNA prep, 96 index
Poly-A-selected total RNA prepared with TruSeq ligation for paired-end sequencing
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model NextSeq 550
 
Data processing QC with FastQC and MultiQC
Quantification with salmon
Quantifications imported with tximport
Normalization and transformed log counts with DESeq2
Assembly: BDGP6.32
Supplementary files format and content: salmon quantification directories as gzip'd archives
Supplementary files format and content: supplementary file contained transformed log counts of all samples
 
Submission date Aug 24, 2023
Last update date Sep 01, 2024
Contact name Jason M Tennessen
E-mail(s) tennessenlab@gmail.com, tennlab@iu.edu, jtenness@iu.edu, tourigny@iu.edu
Phone 8128559803
Organization name Indiana University
Department Biology
Lab Tennessen
Street address 1001 E 3rd St
City Bloomington
State/province IN
ZIP/Postal code 47401
Country USA
 
Platform ID GPL22106
Series (1)
GSE241662 Transcriptomics of impaired hypoxic recovery of L2HGDH mutants
Relations
BioSample SAMN37145617
SRA SRX21480231

Supplementary file Size Download File type/resource
GSM7732864_GSF2857_NM03_14-plus_normox_repl_3.tar.gz 7.7 Mb (ftp)(http) TAR
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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