|
| Status |
Public on Sep 20, 2023 |
| Title |
SHAM surgery, PBS injection, D2 |
| Sample type |
RNA |
| |
|
| Source name |
Gastrocnemius
|
| Organism |
Mus musculus |
| Characteristics |
gender: Female
strain: C57Bl/6
tissue: muscle
sample: Pool of 3 independant samples
|
| Treatment protocol |
Total RNAs were extracted from NHOs mice gastrocnemius biopsy previously flash frozen in isopentane 2 or 4 days after SHAM/NHOs surgery.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted following a chloroform/isopropanol extraction/precipitation protocol
|
| Label |
Sybr Green
|
| Label protocol |
Reverse transcriptions were performed with miRCURY LNA RT kit (339340, Qiagen). Post-RT cDNAs were diluted before microRNAs screening using miRNome Mouse&Rat panel I+II (V5, 339322, Qiagen).
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
Control D2
SHAM PBS D2
|
| Data processing |
Data were obtained on a LightCycler480 (Roche) and processed with LightCycler480 Software. The three most stable microRNAs were selected using Genorm software (v3.4). MiRNAs expressions were quantified using 2ΔCt method (ΔCt = Ctreference - Cttarget) with each reference. The final quantified value is the geometric mean of the 3 values obtained. For in vivo screening, the most stable miRNAs were hsa-miR-24-3p, hsa-miR-23b-3p, hsa-miR-125b-5p. "Matrix non-normalized Template" represents raw Ct values. "Matrix normalized Template" represents normalized values as previously described. "Fold Change Template" represents the ratio between "SHAM PBS D2" and subsequent conditions.
"Matrix non-normalized Template" represents raw Ct values. "Matrix normalized Template" represents normalized values as previously described. "Fold Change Template" represents the ratio between "SHAM PBS D2" and subsequent conditions.
|
| |
|
| Submission date |
Aug 21, 2023 |
| Last update date |
Sep 20, 2023 |
| Contact name |
Jules GUEGUEN |
| E-mail(s) |
julesgueguen1995@gmail.com
|
| Organization name |
French Armed Forces Biomedical Research Institute (IRBA)
|
| Street address |
1 Rue Lieutenant Raoul Batany
|
| City |
Clamart |
| ZIP/Postal code |
92140 |
| Country |
France |
| |
|
| Platform ID |
GPL33702 |
| Series (1) |
| GSE241333 |
Real-time quantitative PCR analysis of microRNAs in a mice model of Neurogenic Heterotopic Ossifications (NHOs) |
|
