|
| Status |
Public on Jul 03, 2024 |
| Title |
HS7666T-DDX18-1 |
| Sample type |
SRA |
| |
|
| Source name |
pancreas
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: pancreas
cell line: Hs766T
cell type: Tumor cell
genotype: ex-DDX18
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Uneasy mini plus kit(Qiagen).
RNA libraries for RNA-seq were prepared using SMARTER mRNA-seq Library Prep Kit following manufacture’s protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Description |
DDX18 overexpressed
|
| Data processing |
CLC Genomics Workbench v11.0.1
Sequence reads weretrimmed for adaptor sequence/low-quality sequence using CLCgeneomic benchwork(parameter-Quality limit:0.05)
Trimmed sequence reads were mapped to GrCM38/mm10 using CLC genomic benchwork(parameters-mismath cost: 2 insertion cost: 3 deletion cost:3 length fraction:0.8 similarity fraction:0.8
Read count extraction and mormalization were performed using CLC genomic benchwork
Supplementary files format and content: mm10
Supplementary files format and content: tab-delimited text files include normalized FPKM values and raw fragment counts for each Sample
|
| |
|
| Submission date |
Aug 21, 2023 |
| Last update date |
Jul 03, 2024 |
| Contact name |
Guoying Dong |
| Organization name |
Shandong University
|
| Department |
Cheeloo College of Medicine
|
| Lab |
chool of Basic Medical Sciences
|
| Street address |
Jinan lixia district Wenhua west road no. 44
|
| City |
Jinan |
| State/province |
Shandong |
| ZIP/Postal code |
250012 |
| Country |
China |
| |
|
| Platform ID |
GPL20795 |
| Series (2) |
| GSE241328 |
The influence of DDX18 high expression in PDAC cells Hs766T on the expression of other intracellular molecules |
| GSE241331 |
Pancreatic cancer |
|
| Relations |
| BioSample |
SAMN37100864 |
| SRA |
SRX21441471 |
