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Sample GSM7720319 Query DataSets for GSM7720319
Status Public on Mar 06, 2024
Title WT_L9
Sample type SRA
Source name Sciatic nerve
Organism Mus musculus
Characteristics tissue: Sciatic nerve
genotype: WT
Extracted molecule total RNA
Extraction protocol Trizol Reagent + Genejet RNA cleanup and Concentration kit (Thermo Scientific)
TruSeq Stranded Total RNA Sample Prep-guide (Part # 15031048 Rev. E)
Total RNAseq after rRNA depletion
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
Data processing raw reads were analyzed for data quality using FastQC v0.11.5 (Babraham Bioinformatics) and eventually filtered using skewer v0.2.2 (Jiang et al., 2014) for removing the low quality reads and trimming the Illumina adapter if necessary.
Mapping of the reads were obtained using the STAR program against human genome (GRCm38) and the quantification of genes and transcripts were done with the RSEM program using GENCODE.vM15 database .
Assembly: Mus musculus (GRCm38)
Supplementary files format and content: RSEM quantification results including Expected counts, TPM and FPKM.
Submission date Aug 21, 2023
Last update date Mar 06, 2024
Contact name Ana Maria Aransay
Phone 0034944061325
Organization name CIC bioGUNE
Department Genome Analysis Platform
Street address Parque tecnologico de Bizkaia, Building 801-A
City Derio
State/province BIZKAIA
ZIP/Postal code 48160
Country Spain
Platform ID GPL24247
Series (1)
GSE241269 Neddylation orchestrates the complex transcriptional and post-transcriptional program that drives axonal myelination
BioSample SAMN37094730
SRA SRX21435735

Supplementary file Size Download File type/resource
GSM7720319_WT_L9.genes.results.gz 1.8 Mb (ftp)(http) RESULTS
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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