|
| Status |
Public on Mar 06, 2024 |
| Title |
WT_L9 |
| Sample type |
SRA |
| |
|
| Source name |
Sciatic nerve
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Sciatic nerve
genotype: WT
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol Reagent + Genejet RNA cleanup and Concentration kit (Thermo Scientific)
TruSeq Stranded Total RNA Sample Prep-guide (Part # 15031048 Rev. E)
Total RNAseq after rRNA depletion
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
raw reads were analyzed for data quality using FastQC v0.11.5 (Babraham Bioinformatics) and eventually filtered using skewer v0.2.2 (Jiang et al., 2014) for removing the low quality reads and trimming the Illumina adapter if necessary.
Mapping of the reads were obtained using the STAR program against human genome (GRCm38) and the quantification of genes and transcripts were done with the RSEM program using GENCODE.vM15 database .
Assembly: Mus musculus (GRCm38)
Supplementary files format and content: RSEM quantification results including Expected counts, TPM and FPKM.
|
| |
|
| Submission date |
Aug 21, 2023 |
| Last update date |
Mar 06, 2024 |
| Contact name |
Ana Maria Aransay |
| E-mail(s) |
amaransay@cicbiogune.es
|
| Phone |
0034944061325
|
| Organization name |
CIC bioGUNE
|
| Department |
Genome Analysis Platform
|
| Street address |
Parque tecnologico de Bizkaia, Building 801-A
|
| City |
Derio |
| State/province |
BIZKAIA |
| ZIP/Postal code |
48160 |
| Country |
Spain |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE241269 |
Neddylation orchestrates the complex transcriptional and post-transcriptional program that drives axonal myelination |
|
| Relations |
| BioSample |
SAMN37094730 |
| SRA |
SRX21435735 |
