|
| Status |
Public on Aug 22, 2023 |
| Title |
Feces, ex-germ free Naïve Feces Recipient [Sample 63] |
| Sample type |
SRA |
| |
|
| Source name |
Feces
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Feces
strain: C57Bl6
treatment: Stress Recipient
Sex: Female
|
| Treatment protocol |
Separately Housed Cd4creAhrfl/fl or Ahrfl/fl
|
| Growth protocol |
Fecal Pellet
|
| Extracted molecule |
total RNA |
| Extraction protocol |
ZymoBIOMICS -96 MagBead DNA kit (Zymo Research, Irvine, CA)
Dual-barcoded primers targeting the 16S V4 region
|
| |
|
| Library strategy |
OTHER |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina MiSeq |
| |
|
| Description |
Zymo Quick-16STM Primer Set V3-V4
63
|
| Data processing |
Demultiplexed reads were preprocessed using DADA2, and the first 20 base pairs were trimmed from forward and reverse reads prior to merging.
Chimeric reads were filtered out, and taxonomic identification was performed with the standard DADA2 classifier using the silva_nr99_v138.1 16S database.
Supplementary files format and content: Csv file with values absolute counts of ASVs by sample
Library strategy: 16S sequencing
|
| |
|
| Submission date |
Aug 17, 2023 |
| Last update date |
Aug 22, 2023 |
| Contact name |
Alban Gaultier |
| E-mail(s) |
ag7h@virginia.edu
|
| Organization name |
University of Virginia
|
| Street address |
409 Lane Rd
|
| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22903 |
| Country |
USA |
| |
|
| Platform ID |
GPL16417 |
| Series (1) |
| GSE241110 |
Lactobacillus from the Altered Schaedler Flora maintain IFNγ homeostasis to promote behavioral stress resilience. |
|
| Relations |
| BioSample |
SAMN37041111 |
| SRA |
SRX21395913 |
