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Sample GSM7716912 Query DataSets for GSM7716912
Status Public on Aug 22, 2023
Title Feces, ex-germ free Naïve Fecese Recipient [Sample 57]
Sample type SRA
 
Source name Feces
Organism Mus musculus
Characteristics tissue: Feces
strain: C57Bl6
treatment: Naive Recipient
Sex: Female
Treatment protocol Separately Housed Cd4creAhrfl/fl or Ahrfl/fl
Growth protocol Fecal Pellet
Extracted molecule total RNA
Extraction protocol ZymoBIOMICS -96 MagBead DNA kit (Zymo Research, Irvine, CA)
Dual-barcoded primers targeting the 16S V4 region
 
Library strategy OTHER
Library source transcriptomic
Library selection other
Instrument model Illumina MiSeq
 
Description Zymo Quick-16STM Primer Set V3-V4
57
Data processing Demultiplexed reads were preprocessed using DADA2, and the first 20 base pairs were trimmed from forward and reverse reads prior to merging.
Chimeric reads were filtered out, and taxonomic identification was performed with the standard DADA2 classifier using the silva_nr99_v138.1 16S database.
Supplementary files format and content: Csv file with values absolute counts of ASVs by sample
Library strategy: 16S sequencing
 
Submission date Aug 17, 2023
Last update date Aug 22, 2023
Contact name Alban Gaultier
E-mail(s) ag7h@virginia.edu
Organization name University of Virginia
Street address 409 Lane Rd
City Charlottesville
State/province VA
ZIP/Postal code 22903
Country USA
 
Platform ID GPL16417
Series (1)
GSE241110 Lactobacillus from the Altered Schaedler Flora maintain IFNγ homeostasis to promote behavioral stress resilience.
Relations
BioSample SAMN37041117
SRA SRX21395907

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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