|
Status |
Public on Mar 24, 2024 |
Title |
4cell_CRxCR_16, mRNA-seq |
Sample type |
SRA |
|
|
Source name |
4 cell embryo
|
Organism |
Mus musculus |
Characteristics |
tissue: 4 cell embryo genotype: WT environment: CRxCR_embryo
|
Extracted molecule |
polyA RNA |
Extraction protocol |
Single embryos, sired by control, germ-free or Rag2−/− sperm or eggs from control or GF mice were collected for single-embryo/egg RNA-sequencing. Embryos were transferred to a 96-well plate and fresh 1 × TCL buffer with 1% β-mercaptoethanol was added. Nextera XT kit (Illumina)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
NextSeq 1000 |
|
|
Description |
data_S1_RNAseq_read_cpm.xlsx
|
Data processing |
Reads were mapped using RSEM and normalized to transcripts per million (tpm). Assembly: mm10 Supplementary files format and content: xlsx spreadsheet with one tab per tissue type/comparison
|
|
|
Submission date |
Aug 14, 2023 |
Last update date |
Mar 24, 2024 |
Contact name |
Natalie Alyce Trigg |
E-mail(s) |
natalie.trigg@newcastle.edu.au
|
Phone |
0249212043
|
Organization name |
Children's Hospital of Philadelphia
|
Lab |
Conine Lab
|
Street address |
421 Curie Blvd
|
City |
Philadelphia |
State/province |
Pennsylvania |
ZIP/Postal code |
19104 |
Country |
USA |
|
|
Platform ID |
GPL32159 |
Series (1) |
GSE240797 |
The microbiota and T cells non-genetically modulate inherited phenotypes transgenerationally |
|
Relations |
BioSample |
SAMN36983418 |
SRA |
SRX21361302 |