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Sample GSM769577 Query DataSets for GSM769577
Status Public on Sep 30, 2011
Title A4gnt null 50W
Sample type RNA
Source name gastric mucosa, A4gnt null, 50 weeks
Organism Mus musculus
Characteristics strain: C57BL/6J
genotype: A4gnt null
age: 50 weeks
tissue: gastric mucosa
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from the gastric mucosa stripped from the muscular layer of the glandular stomach using the RNeasy kit (Qiagen, Hilden, Germany).
Label Cy3
Label protocol Cy3-labeled cRNA was synthesized using the Quick Amp Labeling kit (Agilent Technologies).
Hybridization protocol 1.65 µg of Cy3-labeled cRNA were heat fragmented and applied to Agilent Whole Murine Genome Oligo ver. 2 (4 x 44K) arrays using a Gene Expression Hybridization kit (Agilent Technologies). Hybridization was carried out at 65ºC for 17 hours, and washing was performed using the Gene Expression Wash Pack (Agilent Technologies).
Scan protocol Slides were scanned with an Agilent Technologies Microarray Scanner, and the images were processed by Feature Extraction software (Agilent Technologies) with background correction to prepare the raw date output files.
Description N_50
Data processing Normalization was carried out using a percentile shift algorithm with parameter 75, and then data filtering was performed, using GeneSpring GX 11.0 software (Agilent Technologies).
Submission date Jul 31, 2011
Last update date Sep 30, 2011
Contact name Jun Nakayama
Organization name Shinshu University
Department Graduate School of Medicine
Lab Molecular Pathology
Street address Asahi 3-1-1
City Matsumoto
ZIP/Postal code 390-8621
Country Japan
Platform ID GPL11202
Series (1)
GSE31074 Essential role of gastric gland mucin in preventing gastric cancer in mice

Data table header descriptions
VALUE Normalized log2 signal intensity

Data table
A_55_P1989846 -6.060171
A_55_P1991598 -7.271853
A_55_P2022211 -1.0918078
A_55_P1980764 -4.200946
A_55_P1964375 0.8218279
A_51_P128876 0.30178547
A_55_P2121042 -7.2643676
A_52_P219230 -6.8541794
A_51_P207591 3.3153086
A_55_P2131920 -5.6949215
A_55_P2404223 -4.0454693
A_55_P2101944 5.638377
A_52_P358860 2.4412413
A_51_P119031 -0.9230623
A_51_P309854 -7.2706985
A_51_P343900 1.6850243
A_51_P234359 -2.2548852
A_51_P487813 3.5345354
A_52_P613977 2.864564
A_55_P1957209 -7.25076

Total number of rows: 39483

Table truncated, full table size 928 Kbytes.

Supplementary file Size Download File type/resource
GSM769577.txt.gz 9.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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