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Sample GSM769576 Query DataSets for GSM769576
Status Public on Sep 30, 2011
Title A4gnt null 10W
Sample type RNA
 
Source name gastric mucosa, A4gnt null, 10 weeks
Organism Mus musculus
Characteristics strain: C57BL/6J
genotype: A4gnt null
age: 10 weeks
tissue: gastric mucosa
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from the gastric mucosa stripped from the muscular layer of the glandular stomach using the RNeasy kit (Qiagen, Hilden, Germany).
Label Cy3
Label protocol Cy3-labeled cRNA was synthesized using the Quick Amp Labeling kit (Agilent Technologies).
 
Hybridization protocol 1.65 µg of Cy3-labeled cRNA were heat fragmented and applied to Agilent Whole Murine Genome Oligo ver. 2 (4 x 44K) arrays using a Gene Expression Hybridization kit (Agilent Technologies). Hybridization was carried out at 65ºC for 17 hours, and washing was performed using the Gene Expression Wash Pack (Agilent Technologies).
Scan protocol Slides were scanned with an Agilent Technologies Microarray Scanner, and the images were processed by Feature Extraction software (Agilent Technologies) with background correction to prepare the raw date output files.
Description N_10
Data processing Normalization was carried out using a percentile shift algorithm with parameter 75, and then data filtering was performed, using GeneSpring GX 11.0 software (Agilent Technologies).
 
Submission date Jul 31, 2011
Last update date Sep 30, 2011
Contact name Jun Nakayama
E-mail(s) jnaka@shinshu-u.ac.jp
Organization name Shinshu University
Department Graduate School of Medicine
Lab Molecular Pathology
Street address Asahi 3-1-1
City Matsumoto
ZIP/Postal code 390-8621
Country Japan
 
Platform ID GPL11202
Series (1)
GSE31074 Essential role of gastric gland mucin in preventing gastric cancer in mice

Data table header descriptions
ID_REF
VALUE Normalized log2 signal intensity

Data table
ID_REF VALUE
A_55_P1989846 -4.998685
A_55_P1991598 -6.6255403
A_55_P2022211 -0.7018485
A_55_P1980764 -4.8056726
A_55_P1964375 1.0204811
A_51_P128876 1.0544214
A_55_P2121042 -5.4421844
A_52_P219230 -6.643222
A_51_P207591 2.0782995
A_55_P2131920 -6.6413975
A_55_P2404223 -4.522315
A_55_P2101944 5.2664385
A_52_P358860 2.3676453
A_51_P119031 -1.0446477
A_51_P309854 -6.6000214
A_51_P343900 3.2704182
A_51_P234359 -0.92375755
A_51_P487813 3.3695984
A_52_P613977 2.8197622
A_55_P1957209 -5.51206

Total number of rows: 39483

Table truncated, full table size 928 Kbytes.




Supplementary file Size Download File type/resource
GSM769576.txt.gz 9.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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