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Sample GSM769575 Query DataSets for GSM769575
Status Public on Sep 30, 2011
Title A4gnt null 5W
Sample type RNA
 
Source name gastric mucosa, A4gnt null, 5 weeks
Organism Mus musculus
Characteristics strain: C57BL/6J
genotype: A4gnt null
age: 5 weeks
tissue: gastric mucosa
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from the gastric mucosa stripped from the muscular layer of the glandular stomach using the RNeasy kit (Qiagen, Hilden, Germany).
Label Cy3
Label protocol Cy3-labeled cRNA was synthesized using the Quick Amp Labeling kit (Agilent Technologies).
 
Hybridization protocol 1.65 µg of Cy3-labeled cRNA were heat fragmented and applied to Agilent Whole Murine Genome Oligo ver. 2 (4 x 44K) arrays using a Gene Expression Hybridization kit (Agilent Technologies). Hybridization was carried out at 65ºC for 17 hours, and washing was performed using the Gene Expression Wash Pack (Agilent Technologies).
Scan protocol Slides were scanned with an Agilent Technologies Microarray Scanner, and the images were processed by Feature Extraction software (Agilent Technologies) with background correction to prepare the raw date output files.
Description N_5
Data processing Normalization was carried out using a percentile shift algorithm with parameter 75, and then data filtering was performed, using GeneSpring GX 11.0 software (Agilent Technologies).
 
Submission date Jul 31, 2011
Last update date Sep 30, 2011
Contact name Jun Nakayama
E-mail(s) jnaka@shinshu-u.ac.jp
Organization name Shinshu University
Department Graduate School of Medicine
Lab Molecular Pathology
Street address Asahi 3-1-1
City Matsumoto
ZIP/Postal code 390-8621
Country Japan
 
Platform ID GPL11202
Series (1)
GSE31074 Essential role of gastric gland mucin in preventing gastric cancer in mice

Data table header descriptions
ID_REF
VALUE Normalized log2 signal intensity

Data table
ID_REF VALUE
A_55_P1989846 -5.729248
A_55_P1991598 -6.4082255
A_55_P2022211 -0.09160423
A_55_P1980764 -5.3305235
A_55_P1964375 1.0692587
A_51_P128876 -0.003285885
A_55_P2121042 -6.100091
A_52_P219230 -6.404474
A_51_P207591 2.199195
A_55_P2131920 -6.40264
A_55_P2404223 -3.2278347
A_55_P2101944 4.558897
A_52_P358860 2.5003881
A_51_P119031 -0.8746824
A_51_P309854 -6.3939943
A_51_P343900 1.2424088
A_51_P234359 -1.944613
A_51_P487813 3.572236
A_52_P613977 3.1188154
A_55_P1957209 -5.534205

Total number of rows: 39483

Table truncated, full table size 928 Kbytes.




Supplementary file Size Download File type/resource
GSM769575.txt.gz 9.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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