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Sample GSM769574 Query DataSets for GSM769574
Status Public on Sep 30, 2011
Title Wild type 50W
Sample type RNA
 
Source name gastric mucosa, wild type, 50 weeks
Organism Mus musculus
Characteristics strain: C57BL/6J
genotype: wild type
age: 50 weeks
tissue: gastric mucosa
Extracted molecule total RNA
Extraction protocol Total RNA was isolated from the gastric mucosa stripped from the muscular layer of the glandular stomach using the RNeasy kit (Qiagen, Hilden, Germany).
Label Cy3
Label protocol Cy3-labeled cRNA was synthesized using the Quick Amp Labeling kit (Agilent Technologies).
 
Hybridization protocol 1.65 µg of Cy3-labeled cRNA were heat fragmented and applied to Agilent Whole Murine Genome Oligo ver. 2 (4 x 44K) arrays using a Gene Expression Hybridization kit (Agilent Technologies). Hybridization was carried out at 65ºC for 17 hours, and washing was performed using the Gene Expression Wash Pack (Agilent Technologies).
Scan protocol Slides were scanned with an Agilent Technologies Microarray Scanner, and the images were processed by Feature Extraction software (Agilent Technologies) with background correction to prepare the raw date output files.
Description W_50
Data processing Normalization was carried out using a percentile shift algorithm with parameter 75, and then data filtering was performed, using GeneSpring GX 11.0 software (Agilent Technologies).
 
Submission date Jul 31, 2011
Last update date Sep 30, 2011
Contact name Jun Nakayama
E-mail(s) jnaka@shinshu-u.ac.jp
Organization name Shinshu University
Department Graduate School of Medicine
Lab Molecular Pathology
Street address Asahi 3-1-1
City Matsumoto
ZIP/Postal code 390-8621
Country Japan
 
Platform ID GPL11202
Series (1)
GSE31074 Essential role of gastric gland mucin in preventing gastric cancer in mice

Data table header descriptions
ID_REF
VALUE Normalized log2 signal intensity

Data table
ID_REF VALUE
A_55_P1989846 -5.883401
A_55_P1991598 -5.998202
A_55_P2022211 -0.55138206
A_55_P1980764 -4.9738283
A_55_P1964375 0.9447489
A_51_P128876 -0.23903608
A_55_P2121042 -4.13221
A_52_P219230 -6.582538
A_51_P207591 0.6701441
A_55_P2131920 -6.3130236
A_55_P2404223 -3.4852982
A_55_P2101944 4.4215813
A_52_P358860 2.189434
A_51_P119031 -1.0258555
A_51_P309854 -6.1766653
A_51_P343900 1.7526989
A_51_P234359 -0.7394023
A_51_P487813 3.1936722
A_52_P613977 2.8580608
A_55_P1957209 -6.5587087

Total number of rows: 39483

Table truncated, full table size 928 Kbytes.




Supplementary file Size Download File type/resource
GSM769574.txt.gz 9.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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