|
| Status |
Public on Nov 07, 2023 |
| Title |
B16_F10 cells, CTRL,rep2 |
| Sample type |
SRA |
| |
|
| Source name |
cell line
|
| Organism |
Mus musculus |
| Characteristics |
tissue: cell line
cell line: B16-F10
cell type: melanoma cells
genotype: WT
|
| Growth protocol |
B16-F10 and A375 cells were maintained in DMEM medium containing 1% Penicillin/Streptomycin and 10% FBS andcultured in 37°C incubator with 5%CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The total RNA from B16-F10 or A375 cells was extracted by trizol(Vazyme) according to manufacturer’s protocol for RNA-seq. For chip-seq, B16-F10 cells were fixed by 1% formaldehyde for 10 minutes. Then, chromatin from cells lysate was digested by Micrococcal Nuclease into DNA fragments between 150-900 bp in length and released by sonication for antibody enrichment.
RNA libraries for RNA-seq were prepared using VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina(Vazyme) following manfacturer's protocl.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
FastQC was used to qualified the reads.
The qualified reads were mapped on mouse genome mm10 by STAR(2.7.10b).
The gene counts were quantified by featureCounts (subread 2.0.1).
Assembly: mm10
|
| |
|
| Submission date |
Aug 07, 2023 |
| Last update date |
Nov 07, 2023 |
| Contact name |
Liyuan Zhou |
| E-mail(s) |
zhouliyaun21@mails.ucas.ac.cn
|
| Organization name |
University of Chinese Academy of Sciences
|
| Street address |
国科大杭州高等研究院
|
| City |
Hangzhou |
| State/province |
杭州市 |
| ZIP/Postal code |
310024 |
| Country |
China |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE240258 |
PRMT1 inhibition activates the interferon pathway to potentiate antitumor immunity and enhance checkpoint blockade efficacy in melanoma |
|
| Relations |
| BioSample |
SAMN36873433 |
| SRA |
SRX21282159 |
